Global secretome analysis of resident cardiac progenitor cells from wild‐type and transgenic heart failure mice: Why ambience matters

Resident cardiac progenitor cells (CPCs) have gained attention in cardiac regenerative medicine primarily due to their paracrine activity. In our current study we determined the role of pathological conditions such as heart failure on the autocrine‐paracrine action of stem cell antigen‐1 (Sca‐1) exp...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular physiology 2019-07, Vol.234 (7), p.10111-10122
Main Authors: Samal, Rasmita, Sappa, Praveen Kumar, Gesell Salazar, Manuela, Wenzel, Kristin, Reinke, Yvonne, Völker, Uwe, Felix, Stephan Burkhard, Hammer, Elke, Könemann, Stephanie
Format: Article
Language:English
Subjects:
Ambience
Angiogenesis
Animals
Autocrine signalling
cardiac progenitor cells
Cell proliferation
Cell Proliferation - physiology
Cells (biology)
Collagen
Conditioning
Congestive heart failure
Disease Models, Animal
Extracellular matrix
extracellular matrix proteins
Female
Heart
Heart failure
Heart Failure - metabolism
Heparan sulfate
in vitro angiogenesis
Male
Mice
Mice, Transgenic
Neovascularization, Physiologic - physiology
Paracrine signalling
Plasminogen activator inhibitors
Progenitor cells
Proteins
Proteoglycans
Proteome
Regenerative medicine
Secretion
Secretome
Spectrometry
Stem cells
Stem Cells - metabolism
Transgenic mice
Versican
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Resident cardiac progenitor cells (CPCs) have gained attention in cardiac regenerative medicine primarily due to their paracrine activity. In our current study we determined the role of pathological conditions such as heart failure on the autocrine‐paracrine action of stem cell antigen‐1 (Sca‐1) expressing CPC. This comparative secretome profiling of Sca‐1+ cells derived from transgenic heart failure (αMHC–cyclin‐T1/Gαq overexpression [Cyc] cells) versus healthy (wild‐type [Wt] cells) mice, achieved via mass‐spectrometric quantification, enabled the identification of over 700 proteins. Our results demonstrate that the heart failure milieu caused a 2‐fold enrichment of extracellular matrix proteins (ECM) like biglycan, versican, collagen XII, and angiogenic factors like heparan sulfate proteoglycan 2, plasminogen activator inhibitor 1 in the secretome. We further elucidated the direct influence of the secretome on the functional behavior of Sca‐1 + cells via in vitro tube forming assay. Secreted factors present in the diseased milieu induced tube formation in Cyc cells (1.7‐fold; p 
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.27677