Effects of different concentration and combinations of cryoprotectants on sperm quality, functional integrity in three Indian horse breeds

Traditionally Glycerol (Gly) is being used as major cryoprotectant and its toxicity could be a reason for the variation on stallion sperm freezability and fertility. In an effort to minimize Gly toxicity alternative cryoprotective agents like Di-methyl Formamide (DMF) have been investigated. The eff...

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Bibliographic Details
Published in:Cryobiology 2019-02, Vol.86, p.52-57
Main Authors: Soni, Yogesh, Talluri, T.R., Kumar, Ashok, Ravi, S.K., Mehta, J.S., Tripathi, B.N.
Format: Article
Language:English
Subjects:
Acrosome - drug effects
Acrosome integrity
Animals
Cell Membrane - drug effects
Cryopreservation
Cryopreservation - veterinary
Cryoprotectant
Cryoprotective Agents - pharmacology
DMF
Formamides - adverse effects
Formamides - pharmacology
Freezing
Glycerol
Glycerol - adverse effects
Glycerol - pharmacology
Horses
Male
Semen - drug effects
Semen Preservation - veterinary
Sperm Motility - drug effects
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Summary:Traditionally Glycerol (Gly) is being used as major cryoprotectant and its toxicity could be a reason for the variation on stallion sperm freezability and fertility. In an effort to minimize Gly toxicity alternative cryoprotective agents like Di-methyl Formamide (DMF) have been investigated. The effect of the cryoprotectant and dose of cryoprotective agent varies from breed to breed and also from stallion to stallion within the same breed. Considering these factors a study was designed to study the effects of Gly and DMF at different concentrations and combinations on the plasma membrane, acrosome and DNA integrity as well as other post thaw seminal characteristics of semen of three Indigenous stallion breeds. In the current study, semen was collected from apparently healthy 4–6 years old 3 Marwari, 3 Manipuri and 3 Zanskari breed stallions. After semen collection and evaluation of fresh semen, each semen sample was extended with semen extender containing different concentrations and combinations of Gly and DMF cryoprotectants (i.e. 5% Gly, 5% DMF, 2% Gly, 2% DMF, 2.5% Gly +2.5% DMF and 1% Gly +1% DMF) and frozen. Post thaw semen evaluation was done on the basis of post thaw motility, live sperm count, hypo osmotic swelling test, acrosomal integrity and DNA integrity. Frozen thawed semen showed that the values of plasma membrane integrity, acrosome integrity and DNA integrity parameters were significantly higher (P 
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2018.12.005