Glutathione S-transferase influences the fecundity of Schistosoma japonicum

•Schistosoma japonicum glutathione S-transferase is higher in eggs and male adult.•The egg shells and tegument and parenchymal areas of the worms expressed the enzyme.•Knockdown of the corresponding mRNA showed a significant effect on egg formation. The aim of this study was to investigate the effec...

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Bibliographic Details
Published in:Acta tropica 2019-03, Vol.191, p.8-12
Main Authors: Tang, Chun-lian, Zhou, Hong-hua, Zhu, Ya-wen, Huang, Jin, Wang, Guo-bo
Format: Article
Language:English
Subjects:
Egg
Glutathione S-transferase
RNA interference
Schistosoma japonicum
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Summary:•Schistosoma japonicum glutathione S-transferase is higher in eggs and male adult.•The egg shells and tegument and parenchymal areas of the worms expressed the enzyme.•Knockdown of the corresponding mRNA showed a significant effect on egg formation. The aim of this study was to investigate the effect of Schistosoma japonicum glutathione S-transferase (SjGST) on the developmental stages of the parasite. We found that the mRNA levels of GST were higher in schistosomula obtained from the host and the eggs than that in other developmental stages. SjGST was mainly distributed in the egg shells, teguments of the worms, and part of the parenchyma of the worms. GST knockdown with RNA interference in S. japonicum worms resulted in a silencing rate higher than 80%. The egg reduction rate (18%) and abnormal egg ratio (28%) were significantly higher (P < 0.05) in the GST-silenced group than in the negative control group. These results indicate that SjGST plays an important role in the fecundity of S. japonicum, specifically in egg formation.
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2018.12.027