Long noncoding RNA MEG3 prevents vascular endothelial cell senescence by impairing miR-128-dependent Girdin downregulation

• Published in: American Journal of Physiology: Cell Physiology 2019-06, Vol.316 (6), p.C830-C843
• Main Authors: Lan, Yong, Li, Yong-Jun, Li, Da-Jun, Li, Peng, Wang, Ji-Yang, Diao, Yong-Peng, Ye, Guo-Dong, Li, Yang-Fang
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Animals; Blood platelets; Blood vessels; Cellular Senescence - physiology; Child; Child, Preschool; Down-Regulation - physiology; Endothelial cells; Female; Human Umbilical Vein Endothelial Cells - metabolism; Humans; Infant; Male; Mice; Microfilament Proteins - metabolism; MicroRNAs - antagonists & inhibitors; MicroRNAs - metabolism; miRNA; Oxidation; Phagocytosis; Platelets; Reactive oxygen species; RNA, Long Noncoding - biosynthesis; RNA, Long Noncoding - metabolism; Senescence; Telomerase; Umbilical vein; Vesicular Transport Proteins - metabolism
• ISSN: 0363-6143; 1522-1563
• DOI: 10.1152/ajpcell.00262.2018

Long noncoding RNAs (lncRNAs) are commonly associated with various biological functions, in which the function of lncRNA maternally expressed gene 3 (MEG3) has been identified in various cancers. Strikingly, an association between MEG3 with microRNAs (miRNAs), mRNAs, and proteins has been reported. This study investigates the role of MEG3 in vascular endothelial cell (VEC) senescence. Expression of Girdin and miR-128 was monitored in the blood vessel samples of young and old mice/healthy volunteers, along with the measurement of human umbilical vein endothelial cells (HUVECs). The relationship between MEG3/Girdin and miR-128 was determined and verified. Loss- and gain-of-function approaches were applied to analyze the regulatory effects of MEG3 on platelet phagocytosis and lipoprotein oxidation of HUVEC membrane. In addition, the effect of MEG3 on HUVEC senescence was evaluated by detection of the reactive oxygen species, telomerase activity, and telomere length. To further analyze the MEG3-mediated regulatory mechanism, miR-128 upregulation and inhibition were introduced into the HUVECs. Downregulated Girdin and upregulated miR-128 were found in the blood vessels of old individuals and old mice, as well as in senescent HUVECs. MEG3 downregulation was found to be capable of inhibiting Girdin but enhancing miR-128 expression. It was also indicated to inhibit platelet phagocytosis and reduce telomerase activity and telomere length, while enhancing lipoprotein oxidation and reactive oxygen species production, which ultimately contributed in preventing and protecting HUEVCs from senescence. These findings provide evidence supporting that MEG3 leads to miR-128 downregulation and Girdin upregulation, which promotes platelet phagocytosis, thus protecting VECs from senescence.