BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration

• Published in: Biochemical and biophysical research communications 2019-01, Vol.509 (1), p.125-132
• Main Authors: Yao, Xiangping, Yu, Taiyong, Xi, Fengxue, Xu, Yanting, Ma, Lu, Pan, Xiaohan, Chen, Shujie, Han, Mulan, Yin, Yulong, Dai, Xiaoshuang, Xu, Guohuan, Zhang, Huabing, Yang, Gongshe, Xie, Liwei
• Format: Article
• Language: English
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2018.12.082

Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) gene encodes a transmembrane protein and is involved in multiple physiological and pathological processes, such as inflammatory response, tumor development and progression, cell proliferation and differentiation. A previous study suggested that BAMBI may interact with the Wnt/β-catenin signaling pathway via promoting β-catenin nuclear translocation associated with C2C12 myogenic myoblast differentiation. However, its biological function in skeletal muscle still remains unknown and requires further characterization. The present work sought to investigate its biological function in skeletal muscle, especially the physiological roles of BAMBI during skeletal muscle growth and regeneration. Our current work suggests that BAMBI protein is highly expressed in skeletal muscle and is only detected in cytosolic fraction in the resting muscle. Moreover, BAMBI protein is co-localized in fast-twitch (glycolytic) fibers, but not in slow-twitch (oxidative) fibers. Comparing with the cytosolic trapping in resting muscle, BAMBI protein is enriched on cellular membrane during the muscle growth and regeneration, suggesting that BAMBI-mediated a significant signaling pathway may be an essential part of muscle growth and regeneration. •BAMBI protein is expressed in cytosol of adult resting skeletal muscle.•BAMBI protein migrates to the cellular membrane of skeletal muscle during development andregeneration.•BAMBI protein is preferentially co-localized in fast-twitch myofibers.