Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats

• Published in: Journal of integrative medicine 2019-01, Vol.17 (1), p.66-70
• Main Authors: Abd Aziz, Che Badariah, Ahmad Suhaimi, Siti Qusyasyiah, Hasim, Hidani, Ahmad, Asma Hayati, Long, Idris, Zakaria, Rahimah
• Format: Article
• Language: English
• Subjects: Animals; Catalase - metabolism; Female; Glutathione - metabolism; Honey - analysis; Male; Malondialdehyde - metabolism; Neurons - metabolism; Nociception; Nociceptive responses; Oxidative Stress; Pregnancy; Pregnancy Complications - diet therapy; Pregnancy Complications - psychology; Prenatal Exposure Delayed Effects - metabolism; Prenatal Exposure Delayed Effects - physiopathology; Prenatal stress; Rats; Rats, Sprague-Dawley; Spinal cord; Spinal Cord - anatomy & histology; Spinal Cord - metabolism; Stress, Psychological - diet therapy; Stress, Psychological - psychology; Superoxide Dismutase - metabolism; Tualang honey
• ISSN: 2095-4964
• DOI: 10.1016/j.joim.2018.12.002

This study was done to determine whether Tualang honey could prevent the altered nociceptive behaviour, with its associated changes of oxidative stress markers and morphology of the spinal cord, among the offspring of prenatally stressed rats. Pregnant rats were divided into three groups: control, stress, and stress treated with Tualang honey. The stress and stress treated with Tualang honey groups were subjected to restraint stress from day 11 of pregnancy until delivery. Ten week old male offspring (n = 9 from each group) were given formalin injection and their nociceptive behaviours were recorded. After 2 h, the rats were sacrificed, and their spinal cords were removed to assess oxidative stress activity and morphology. Nociceptive behaviour was analysed using repeated measures analysis of variance (ANOVA), while the levels of oxidative stress parameters and number of Nissl-stained neurons were analysed using a one-way ANOVA. This study demonstrated that prenatal stress was associated with increased nociceptive behaviour, changes in the oxidative stress parameters and morphology of the spinal cord of offspring exposed to prenatal stress; administration of Tualang honey reduced the alteration of these parameters. This study provides a preliminary understanding of the beneficial effects of Tualang honey against the changes in oxidative stress and neuronal damage in the spinal cord of the offspring of prenatally stressed rats.