Separating the contributions of zona pellucida and cytoplasm in the viscoelastic response of human oocytes

• Published in: Acta biomaterialia 2019-02, Vol.85, p.253-262
• Main Authors: Shen, Tong, Benet, Eduard, Sridhar, Shankar Lalitha, Abadie, Joel, Piat, Emmanuel, Vernerey, Franck J.
• Format: Article
• Language: English
• Subjects: Compression/indentation tests; Cytoplasm; Cytoplasm - metabolism; Elasticity; Embryos; Experimental protocol; Finite Element Analysis; Heterogeneity; Human behavior; Humans; In vitro fertilization; Mechanical properties; Models, Biological; Modified Hertz contact; Oocyte; Oocytes; Oocytes - metabolism; Pregnancy; Relaxation time; Reproducibility of Results; Reproductive technologies; Risk reduction; Stiffness; Stress relaxation; Test procedures; Viscoelasticity; Viscosity; Zona pellucida; Zona Pellucida - metabolism
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2018.12.034

[Display omitted] The successful characterization of the mechanical properties of human oocytes and young embryos is of crucial relevance to reduce the risk of pregnancy arrest in in-vitro fertilization processes. Unfortunately, current study has been hindered by the lack of accuracy in describing the mechanical contributions of each structure (zona pellucida, cytoplasm) due to its high heterogeneity. In this work, we present a novel approach to model the oocyte response taking into account the effect of both zona and cytoplasm, as well as different loading conditions. The model is then applied to develop an experimental protocol capable of accurately separating the viscoelastic contribution of zona and cytoplasm by simply varying the loading condition. This new protocol has the potential to open the door to improving our understanding the mechanical properties of oocytes at different stages, and provide a quantitative predictive ability to the evaluation of oocyte quality. Assisted reproductive technologies, such as in vitro fertilization, often rely on identifying high quality oocytes or female egg cells. The viscoelastic properties of these cells, such as stiffness and stress relaxation time, have been identified as potential objective indicators of cell quality. However, their characterization has proven difficult due to the structural heterogeneity of the cell and inconsistent loading conditions. This paper presents a new model that, although simple, addresses the above difficulties to provide accurate estimations of the cell’s mechanical properties. Learning from this model, we then propose a novel non-invasive testing protocol to allow oocyte characterization with increased accuracy. We believe this effort would improve consistency in measurements and enhance our knowledge on the mechanics of oocytes.