Dietary nucleotides can directly stimulate the immunity of zebrafish independent of the intestinal microbiota

In this study, we firstly tested the effects of dietary nucleotides on the disease resistance and innate immunity of zebrafish. Further, we investigated the role of intestinal microbiota in the nucleotides-induced immunostimulatory effect by using a germ-free zebrafish model and microbiota transfer...

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Bibliographic Details
Published in:Fish & shellfish immunology 2019-03, Vol.86, p.1064-1071
Main Authors: Guo, Xiaoze, Li, Jie, Ran, Chao, Wang, Anran, Xie, Mingxu, Xie, Yadong, Ding, Qianwen, Zhang, Zhen, Yang, Yalin, Duan, Ming, Zhou, Zhigang
Format: Article
Language:English
Subjects:
Innate immune response
Intestinal microbiota
Nucleotides
Zebrafish
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Summary:In this study, we firstly tested the effects of dietary nucleotides on the disease resistance and innate immunity of zebrafish. Further, we investigated the role of intestinal microbiota in the nucleotides-induced immunostimulatory effect by using a germ-free zebrafish model and microbiota transfer technique. Fish were fed control or nucleotides (NT)-supplemented diets (at 0.05%,0.1%, 0.15% or 0.2%, m/m) for 4 weeks, followed by immersion challenge with Aeromonas hydrophila NJ-1. The results showed that 0.1% NT group enhanced the resistance of zebrafish against A. hydrophila infection. We further observed that the relative expressions of mucin, claudin16, occlusin1, hepcidin, defensin beta-like, myeloperoxidase (Mpo), and serum amyloid A (Saa) increased in the 0.1% NT group compared with control (P 
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2018.12.058