Automated assays for von Willebrand factor activity

von Willebrand factor (VWF) ristocetin cofactor activity (VWF:RCo) by platelet aggregometry has been considered the gold standard for evaluating the ability of VWF to bind platelets for over 40 years. Many automated systems no longer require platelets and rather rely on agglutination of latex partic...

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Bibliographic Details
Published in:American journal of hematology 2019-04, Vol.94 (4), p.496-503
Main Authors: Higgins, Russell A., Goodwin, Andrew J.
Format: Article
Language:English
Subjects:
Agglutination
Automation
Automation, Laboratory
Blood Coagulation Tests - history
Blood Coagulation Tests - instrumentation
Blood Coagulation Tests - methods
Blood Platelets - metabolism
Chemiluminescence
Health risk assessment
Hematology
History, 20th Century
History, 21st Century
Humans
Latex
Platelets
Ristocetin
Von Willebrand factor
von Willebrand Factor - history
von Willebrand Factor - metabolism
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Summary:von Willebrand factor (VWF) ristocetin cofactor activity (VWF:RCo) by platelet aggregometry has been considered the gold standard for evaluating the ability of VWF to bind platelets for over 40 years. Many automated systems no longer require platelets and rather rely on agglutination of latex particles. Automated methods of measuring VWF activity have improved performance characteristics and are performed on the same coagulation instruments used for routine testing via immunoturbidimetric methodology. Alternatively, a newer chemiluminescence assay system for measuring VWF activity demonstrates excellent performance characteristics. As these methods are becoming widely used, it is important to assess their performance in diagnosing and monitoring different types of von Willebrand disease. We review the automated methodologies and the published performance of these VWF assays. Advantages and limitations of these automated methods are discussed.
ISSN:0361-8609
1096-8652
DOI:10.1002/ajh.25393