Prognostic value of initial bone marrow disease detection by multiparameter flow cytometry in children with neuroblastoma

Purpose Multicolor flow cytometry (MFC) is widely available, fast and has an easy-to perform approach for finding neuroblastoma (NB) cells among normal bone marrow (BM) hematopoietic cells. Aim of the study was to investigate prognostic significance of initial MFC tumor cells’ detection in BM of chi...

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Bibliographic Details
Published in:Journal of cancer research and clinical oncology 2019-02, Vol.145 (2), p.535-542
Main Authors: Popov, Alexander, Druy, Alexander, Shorikov, Egor, Verzhbitskaya, Tatiana, Solodovnikov, Alexander, Saveliev, Leonid, Tytgat, Godelieve A. M., Tsaur, Grigory, Fechina, Larisa
Format: Article
Language:English
Subjects:
Bone marrow
Cancer Research
CD45 antigen
CD56 antigen
CD81 antigen
Children
Flow cytometry
Hematology
Internal Medicine
Medicine
Medicine & Public Health
Multivariate analysis
Neuroblastoma
Oncology
Original Article – Clinical Oncology
Patients
Phox2b protein
Risk factors
Tumor cells
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Summary:Purpose Multicolor flow cytometry (MFC) is widely available, fast and has an easy-to perform approach for finding neuroblastoma (NB) cells among normal bone marrow (BM) hematopoietic cells. Aim of the study was to investigate prognostic significance of initial MFC tumor cells’ detection in BM of children with NB. Methods 51 patients (24 boys and 27 girls) aged from 6 days to 15 years (median age 1 year 3 months) with NB were included in the study. BM samples at the time of diagnosis were obtained from 2 to 5 aspiration sites per patient. CD45(−)CD56(+)CD81(+)GD2(+)-cells were evaluated by MFC. Results NB cells were detected in BM by FC more frequently compared to conventional cytomorphology (49.0% and 29.4% patients, respectively, р  = 0.043). Patients with NB cells detected in BM by MFC had significantly worse event-free survival and cumulative incidence of relapse/progression [0.24(0.08) and 0.60(0.10), respectively] compared to children with negative result of immunophenotyping [0.85(0.07) and 0.12(0.06), respectively, p  
ISSN:0171-5216
1432-1335
DOI:10.1007/s00432-018-02831-w