The appropriate remodeling of extracellular matrix is the key molecular signature in subcutaneous adipose tissue following Roux-en-Y gastric bypass

• Published in: Life sciences (1973) 2019-02, Vol.218, p.265-273
• Main Authors: Chen, Xiangjun, Gong, Lilin, Li, Qifu, Hu, Jinbo, Liu, Xiurong, Wang, Yao, Bai, Jie, Ran, Xi, Wu, Jinshan, Ge, Qian, Li, Rong, Xiao, Xiaoqiu, Li, Xi, Zhang, Jun, Wang, Zhihong
• Format: Article
• Language: English
• Subjects: Adipose tissue; Animals; Bioinformatics; Biomarkers - metabolism; Collagen (type I); Computational Biology; Cross-linking; Crosslinking; Encyclopedias; Extracellular matrix; Extracellular Matrix - metabolism; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - metabolism; Extracellular matrix remodeling; Gastric Bypass; Gene expression; Genes; Genomes; In vivo methods and tests; Insulin; Lysyl oxidase; Male; Oxidase; Protein interaction; Protein Interaction Maps; Proteins; Rats; Rats, Sprague-Dawley; Rodents; Roux-en-Y gastric bypass; Subcutaneous adipose tissue; Subcutaneous Fat - metabolism; Subcutaneous Fat - surgery; Transcriptome; Transcriptomics
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2018.12.051

We sought to reveal the key molecular signature in subcutaneous adipose tissue (scAT) following Roux-en-Y gastric bypass (RYGB), through bioinformatics analysis and further verification in vivo. We obtained a transcriptome data of scAT from RYGB and sham-operated rats from the Gene Expression Omnibus. The differentially expressed genes (DEGs) were screened and the DEGs-related Gene ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were analyzed. Also, the protein-protein interaction (PPI) network was constructed among the DEGs. Furthermore, we established an experimental rat model to verify the bioinformatics findings. Using the method of bioinformatics, a total of 602 genes were found to be differentially expressed in scAT between the RYGB group and the sham-operated group. GO analysis showed that DEGs were significantly enriched in extracellular matrix(ECM) -associated functions or processes. KEGG pathway analysis revealed that the protein digestion and absorption pathway and ECM-receptor interaction pathway were the most significantly enriched pathways. The genes encoding ECM components and ECM remodeling-related proteins interact substantially in the PPI network. Then the results of rat experimental verified that the gene expression levels of ECM components(Collagen I and III) and ECM cross-linking related proteins(lysyl oxidase and lysyl oxidase-like 1) decreased and ECM dagradation-related proteins increased in scAT following RYGB. These beneficial results were positively associated with improved insulin resistance (IR). Appropriate ECM remodeling, primarily the reduction of ECM deposition and cross-linking and the increase of ECM dagradation, may be the key molecular signature in scAT following RYGB.