A novel voltammetry offline coupled MALDI/TOF MS characterization of electrochemical reaction products and the voltammetric determination of febuxostat in human plasma

A simple offline coupling voltammetry-MALDI/TOF MS procedure is presented for studying electrochemical reactions. It was utilized for the characterization of the electro-reduction products of febuxostat in methanolic acetate buffer (0.1 M, pH 5). The MS analysis reveals that the carboxylic and nitri...

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Bibliographic Details
Published in:Talanta (Oxford) 2019-03, Vol.194, p.542-547
Main Authors: El-Yazbi, Fawzi A., Amin, Omayma A., Bakry, Rania, Khamis, Essam F., El-Kimary, Eman I., Younis, Sameh E.
Format: Article
Language:English
Subjects:
Febuxostat
Human plasma
MALDI/TOF MS
Tablets
Voltammetry
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Summary:A simple offline coupling voltammetry-MALDI/TOF MS procedure is presented for studying electrochemical reactions. It was utilized for the characterization of the electro-reduction products of febuxostat in methanolic acetate buffer (0.1 M, pH 5). The MS analysis reveals that the carboxylic and nitrile groups are the electro-reducible groups at −0.9338 and −1.5503 V with the conversion to aldehydic and amino groups, respectively. The developed voltammetric method was validated and applied successfully for the drug determination in pharmaceutical tablets and real plasma samples within the linearity ranges 0.03–2 and 0.4–5 μg mL−1, respectively. [Display omitted] •Novel off line coupling voltammetry-MALDI/TOF procedure for studying electrochemical reactions.•Characterization of the electro-reduction products of febuxostat in methanolic acetate buffer.•Reduction of carboxylic and nitrile groups to aldehydic and amino groups.•Voltammetric determination of febuxostat in dosage form and real human plasma.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2018.10.087