The Effect of Parietin Isolated From Rheum ribes L on In Vitro Wound Model Using Human Dermal Fibroblast Cells

• Published in: International journal of lower extremity wounds 2019-03, Vol.18 (1), p.56-64
• Main Authors: Gundogdu, Gulsah, Gundogdu, Koksal, Nalci, Kemal Alp, Demirkaya, Alper Kursat, Yılmaz Tascı, Seymanur, Demirkaya Miloglu, Fatma, Senol, Onur, Hacimuftuoglu, Ahmet
• Format: Article
• Language: English
• Subjects: Cell Proliferation - drug effects; Cells, Cultured; Emodin - analogs & derivatives; Emodin - pharmacology; Fibroblasts - drug effects; Humans; In Vitro Techniques; Rheum; Ribes; Sensitivity and Specificity; Wound Healing - drug effects; Wound Healing - physiology; Wounds and Injuries - therapy
• ISSN: 1534-7346; 1552-6941
• DOI: 10.1177/1534734618819660

Parietin is one of the well-known anthraquinone compounds that can be extracted from Rheum ribes L. In this study, we aimed to investigate the effects of parietin isolated from Rheum ribes L on an in vitro wound model using human dermal fibroblast cells and compare its effectiveness against zinc. The antioxidant effect of parietin was determined by using the 1,1-diphenyl-2-picrylhydrazine (DPPH) method. Human dermal fibroblast cells were cultured in proculture medium and were kept until 100% confluence was achieved. The wound model was created by using a pipette tip. After that, different concentrations of parietin and zinc (final concentrations in the well to be 5-250 µM and 25-200 µM, respectively) were added into the medium. The proliferation-inducing effect on cell viability was determined by using MTT assay. Images of cells were taken at 0, 12, and 24 hours. According to the DPPH method, parietin exhibited have antioxidant activity. According to the MTT results, parietin exhibited significant proliferation-inducing effect on cell viability in a dose range of 5 to 10 M, and zinc showed significant proliferation-inducing effect on cell viability at dose 50 µM (P < .05). In addition, the image of cell proliferation was also shown at the same doses at 24 hours. In this study, we claim that parietin induces cell proliferation at low doses in cases of dermal fibroblast loss. In conclusion, parietin as an alternative to zinc in wound healing could be used by clinicians in the future with more extensive studies.