The Effects of Probiotic Supplements on Blood Markers of Endotoxin and Lipid Peroxidation in Patients Undergoing Gastric Bypass Surgery; a Randomized, Double-Blind, Placebo-Controlled, Clinical Trial with 13 Months Follow-Up

• Published in: Obesity surgery 2019-04, Vol.29 (4), p.1248-1258
• Main Authors: Mokhtari, Zeinab, Karbaschian, Zohreh, Pazouki, Abdolreza, Kabir, Ali, Hedayati, Mehdi, Mirmiran, Parvin, Hekmatdoost, Azita
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Biomarkers - blood; Clinical trials; Combined Modality Therapy; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - drug therapy; Diabetes Mellitus, Type 2 - surgery; Dietary Supplements; Double-Blind Method; Double-blind studies; Endotoxins - blood; Female; Follow-Up Studies; Gastric Bypass - methods; Gastrointestinal surgery; Humans; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipid Peroxidation - physiology; Male; Medicine; Medicine & Public Health; Middle Aged; Obesity, Morbid - blood; Obesity, Morbid - complications; Obesity, Morbid - drug therapy; Obesity, Morbid - surgery; Original Contributions; Placebos; Probiotics; Probiotics - therapeutic use; Surgery; Treatment Outcome; Weight Loss - drug effects; Young Adult
• ISSN: 0960-8923; 1708-0428
• DOI: 10.1007/s11695-018-03667-6

Background The effect of probiotic supplements among subjects undergoing bariatric surgery indicates conflicting results. Moreover, whether these effects remain after ceasing the treatment remained to be elucidated. This study was conducted to assess the effect of probiotic supplements on blood markers of endotoxin (lipopolysaccharides-binding protein: LBP), inflammation and lipid peroxidation (malondialdehyde: MDA) in patients with morbid obesity undergoing the one-anastomosis gastric bypass (OAGB). Methods This study is a placebo-controlled, double-blind, and randomized clinical trial and 9 months of additional follow-up. Forty-six morbid obese patients undergoing OAGB were randomized to 4 months of probiotic or placebo supplements. Anthropometric indices and blood concentration of LBP, inflammatory markers, MDA, vitamin D3, and B 12 were measured at 0, 4, and 13 months of study. Results Probiotic supplements could improve serum LBP ( P  = 0.039), TNF-α ( P  = 0.005), vitamin B 12 ( P  = 0.03), vitamin D3 ( P  = 0.001), and weight loss ( P  = 0.01) at month 4 in comparison to placebo; however, only serum MDA concentrations decreased significantly in the probiotic group compared with those in the placebo group ( P  = 0.013) at the end of follow-up period. Discussion It was observed that 4 months probiotic supplementation compared with placebo prohibited an elevation in the LBP levels and improved serum TNF-α and 25-OH vitamin D3 concentrations and weight loss in patients undergoing the OAGB surgery. However, these effects did not persist 9 months after the cessation of the treatment. Further investigations are required to find how long supplementation and which dosage of it can benefit body status for the long-term. Trial Registration This study has been registered at Clinicaltrial.gov with registration number NCT02708589 .