Type IV chitinase quantification in four different grape cultivars (Vitis vinifera) in northeast of Iran by an indirect sandwich enzyme-linked immunosorbent assay

The incidence of grape (Vitis vinifera) allergy in the northeast of Iran is second to melon allergy. Type IV chitinase is one of the major grape allergens. The current study investigates the level of type IV chitinase in four grape variants for the first time in Khorasan Razavi Province using a high...

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Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2019-03, Vol.40 (2), p.139-148
Main Authors: Farshidi, Narges, Moghaddam, Maliheh, Yaghoubi, Vahid, Ayati, Seyed Hasan, Varasteh, Abdol-reza, Sankian, Mojtaba
Format: Article
Language:English
Subjects:
Allergens
Allergens - analysis
Allergens - immunology
Allergies
Antibodies - immunology
Antigen-Antibody Reactions
Assaying
Chitinase
Chitinases - analysis
Chitinases - immunology
Cultivars
ELISA
Enzyme-Linked Immunosorbent Assay
Enzymes
Grape (vitis vinifera)
Grapes
indirect sandwich enzyme-linked immunosorbent assay
Iran
Monoclonal antibodies
pathogenesis-related proteins
type IV chitinase
Vitis - chemistry
Vitis - immunology
Vitis vinifera
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Summary:The incidence of grape (Vitis vinifera) allergy in the northeast of Iran is second to melon allergy. Type IV chitinase is one of the major grape allergens. The current study investigates the level of type IV chitinase in four grape variants for the first time in Khorasan Razavi Province using a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA). This assay was developed using a polyclonal antibody as a capture antibody and monoclonal antibody as a secondary one. Finally, the amount of type IV chitinase was measured by the validated ELISA test. The sensitivity of the developed sandwich ELISA is 16 ± 0.05 ng/ml, and its mean coefficients of intraday and interday variations are
ISSN:1532-1819
1532-4230
DOI:10.1080/15321819.2018.1535439