Repeated repeat problems: Combinatorial effect of C9orf72-derived dipeptide repeat proteins

A microsatellite expansion mutation in C9orf72 is the most common genetic cause of Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD). The expansion mutation leads to C9orf72 loss of function, RNA foci formation, and generation of five species of non-AUG RAN translated dipeptide r...

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Bibliographic Details
Published in:International journal of biological macromolecules 2019-04, Vol.127, p.136-145
Main Authors: Darling, April L., Breydo, Leonid, Rivas, Emma G., Gebru, Niad T., Zheng, Dali, Baker, Jeremy D., Blair, Laura J., Dickey, Chad A., Koren, John, Uversky, Vladimir N.
Format: Article
Language:English
Subjects:
ALS
C9orf72
Dipeptide repeat protein
FTD
Intrinsically disordered proteins
Poly(GA)
Poly(PR)
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Summary:A microsatellite expansion mutation in C9orf72 is the most common genetic cause of Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD). The expansion mutation leads to C9orf72 loss of function, RNA foci formation, and generation of five species of non-AUG RAN translated dipeptide repeat proteins (DPRs), such as poly(GA), poly(GP), poly(GR), poly(PA), and poly(PR). Although one cell can contain more than type of DPRs, information about interplay between different DPR species is limited. Here we show that the combined expression of distinct C9orf72-derived dipeptide repeat species produces cellular outcomes and structural differences that are unique compared to the expression of a single DPR species, suggesting the complex biological interactions that occur when multiple DPR variants are simultaneously expressed. Our data highlights the need for further analysis of how combined expression of different DPRs affects the disease state.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.01.035