Rn7SK small nuclear RNA is involved in cellular senescence

• Published in: Journal of cellular physiology 2019-08, Vol.234 (8), p.14234-14245
• Main Authors: Musavi, Maryam, Kohram, Fatemeh, Abasi, Mozhgan, Bolandi, Zohreh, Ajoudanian, Mohammad, Mohammadi‐Yeganeh, Samira, Hashemi, Seyed Mahmoud, Sharifi, Kazem, Fathi, Hamid reza, Ghanbarian, Hossein
• Format: Article
• Language: English
• Subjects: Adipose tissue; Aging; Aging - genetics; beta-Galactosidase - genetics; Biocompatibility; Biomedical materials; Cell Differentiation - genetics; Cell Proliferation - genetics; Cell Survival - genetics; Cell viability; Cellular Senescence - genetics; Differentiation (biology); Galactosidase; Humans; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Mesenchyme; noncoding RNAs; Osteogenesis - genetics; Regenerative Medicine; Ribonucleic acid; Rn7SK; RNA; RNA, Small Nuclear - genetics; Senescence; Signal Transduction - genetics; snRNA; Stem cells; Stem Cells - metabolism; Tissue engineering; Transfection; Tumor cell lines; β-Galactosidase
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.28119

Rn7SK is a conserved small nuclear noncoding RNA which its function in aging has not been studied. Recently, we have demonstrated that Rn7SK overexpression reduces cell viability and is significantly downregulated in stem cells, human tumor tissues, and cell lines. In this study, we analyzed the role of Rn7SK on senescence in adipose tissue‐derived mesenchymal stem cells (AD‐MSCs). For this purpose, Rn7SK expression was downregulated and upregulated via transfection and transduction, respectively, in AD‐MSCs and subsequently, various distinct characteristics of senescence including cell viability, proliferation, colony formation, senescence‐associated β galactosidase activity, and differentiation potency was analyzed. Our results demonstrated the transient knockdown of Rn7SK in MSCs leads to delayed senescence, while its overexpressions shows opposite effects. When osteogenic differentiation was started, however, they exhibited a greater differentiation potential than the original MSCs, suggesting a potential tool for stem cell‐based regenerative medicine. In this study, we analyzed the role of Rn7SK on senescence in adipose tissue‐derived mesenchymal stem cells (AD‐MSCs). Our results demonstrated the transient knockdown of Rn7SK in MSCs leads to a delayed senescence, while its overexpressions shows opposite effects.