Separation of anti-ulcer flavonoids from Artemisia extracts by high-speed countercurrent chromatography

• Published in: Food chemistry 2011-11, Vol.129 (2), p.679-683
• Main Authors: Yoon, Kee Dong, Chin, Young-Won, Yang, Min Hye, Kim, Jinwoong
• Format: Article
• Language: English
• Subjects: acetates; anti-ulcer activity; Anti-ulcer flavonoids; Artemisia absinthium; Artemisia indica var. maximowiczii; Artemisia princeps; Biological and medical sciences; countercurrent chromatography; ethanol; Eupatilin; flavonoids; Food industries; Fundamental and applied biological sciences. Psychology; HSCCC; Jaceosidin; plant extracts; separation
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2011.05.005

► HSCCC separates anti-ulcer flavonoids in one step. ► HSCCC leads to isolation of eupatilin and jaceosidin with high yield and purity. ► The separation efficiency of HSCCC was superior to that of conventional methods. High-speed countercurrent chromatography (HSCCC) was applied to separate two anti-ulcer flavonoids, eupatilin (5,7-dihydroxy-6,3′,4′-trimethoxyflavone, 1) and jaceosidin (5,7,4′-trihydroxy-6,3′-dimethoxyflavone, 2), isolated from the ethanol, ethyl acetate and semi-purified ethanol extract of dietary wormwood (Artemisia princeps). The two phase solvent system comprising n-hexane–chloroform–methanol–water (2:5:5:2, v/v/v/v) was employed to HSCCC and the flow rate of mobile phase was optimised at 1.3ml/min. Utilising this method, the recovery rate and purity of eupatilin (1) and jaceosidin (2) separated from three Artemisia extracts were evaluated to be over 95% and 90%, respectively. These results suggest that countercurrent separation method can be applied to isolate two anti-ulcer flavonoids, eupatilin (1) and jaceosidin (2), selectively in one step, and to produce anti-ulcer flavonoid-enriched preparations.