Age related changes in cell stiffness of tendon stem/progenitor cells and a rejuvenating effect of ROCK-inhibition

Tendon stem/progenitor cells (TSPC) are potential targets for regenerative medicine and the treatment of tendon injuries. The frequency of such injuries increases in elderly patients while the proportion of functional TSPCs in tendon tissue decreases, protracting tendon repair. Using atomic force mi...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2019-02, Vol.509 (3), p.839-844
Main Authors: Kiderlen, Stefanie, Polzer, Christoph, Rädler, Joachim O., Docheva, Denitsa, Clausen-Schaumann, Hauke, Sudhop, Stefanie
Format: Article
Language:English
Subjects:
Cell aging
Elastic modulus
hTSPC
Rejuvenation
ROCK-inhibition
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tendon stem/progenitor cells (TSPC) are potential targets for regenerative medicine and the treatment of tendon injuries. The frequency of such injuries increases in elderly patients while the proportion of functional TSPCs in tendon tissue decreases, protracting tendon repair. Using atomic force microscopy (AFM), we show that cell stiffness and size increase in TSPCs isolated from elderly patients (A-TSPC) compared to TSPCs from younger patients (Y-TSPC). Additionally, two-photon excited fluorescence (TPEF) microscopy revealed a denser, well-structured actin cytoskeleton in A-TSPC, which correlates with the augmented cell stiffness. Treating A-TSPC with ROCK-inhibitor, reverses these age-related changes, and has rejuvenating effect on cell morphology and stiffness. We assume that cellular stiffness is a suitable marker for cell aging and ROCK a potential target for therapeutic applications of cell rejuvenation. [Display omitted] •Stiffness and size of tendon stem/progenitor cells (TSPC) increase with age.•Increased cell stiffness correlates with denser and oriented actin fibers.•ROCK inhibition reverses aging-effects having a rejuvenating effect on aged TSPC.•ROCK may serve as target and cell stiffness as biomarker in cell based therapies.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2019.01.027