Mass spectrometry‐based proteomics reveals the distinct nature of the skin proteomes of photoaged compared to intrinsically aged skin

• Published in: International journal of cosmetic science 2019-04, Vol.41 (2), p.118-131
• Main Authors: Newton, V.L., Riba‐Garcia, I., Griffiths, C.E.M., Rawlings, A.V., Voegeli, R., Unwin, R.D., Sherratt, M.J., Watson, R.E.B.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Age; Aged; Aging; Biopsy; dermal–epidermal junction; Dermis; Energy metabolism; Epidermis; Female; Forearm; Gel electrophoresis; Gene expression; genomics/proteomics/ELISA/cell culture; Humans; Liquid chromatography; Male; Mass spectrometry; Mass Spectrometry - methods; Mass spectroscopy; Metabolism; Middle Aged; Protein biosynthesis; Protein synthesis; Proteins; Proteomics; Scavenging; Scientific imaging; Skin; Skin - metabolism; Skin - radiation effects; skin ageing; Skin Aging; skin physiology/structure; Sodium lauryl sulfate; Spectroscopy; Stratum corneum; Young Adult
• ISSN: 0142-5463; 1468-2494
• DOI: 10.1111/ics.12513

Objective With increasing age, skin is subject to alterations in its organization, which impact on its function as well as having clinical consequences. Proteomics is a useful tool for non‐targeted, semi‐quantitative simultaneous investigation of high numbers of proteins. In the current study, we utilize proteomics to characterize and contrast age‐associated differences in photoexposed and photoprotected skin, with a focus on the epidermis, dermal–epidermal junction and papillary dermis. Methods Skin biopsies from buttock (photoprotected) and forearm (photoexposed) of healthy volunteers (aged 18–30 or ≥65 years) were transversely sectioned from the stratum corneum to a depth of 250 μm. Following SDS‐PAGE, each sample lane was segmented prior to analysis by liquid chromatography‐mass spectrometry/mass spectrometry. Pathway analysis was carried out using Ingenuity IPA. Results Comparison of skin proteomes at buttock and forearm sites revealed differences in relative protein abundance. Ageing in skin on the photoexposed forearm resulted in 80% of the altered proteins being increased with age, in contrast to the photoprotected buttock where 74% of altered proteins with age were reduced. Functionally, age‐altered proteins in the photoexposed forearm were associated with conferring structure, energy and metabolism. In the photoprotected buttock, proteins associated with gene expression, free‐radical scavenging, protein synthesis and protein degradation were most frequently altered. Conclusion This study highlights the necessity of not considering photoageing as an accelerated intrinsic ageing, but as a distinct physiological process. Résumé Objectif Avec l’âge, la peau est sujette à des altérations dans son organisation, et outre le fait d'avoir des conséquences cliniques cela a un impact sur sa fonction. La protéomique est un outil utile pour l’évaluation non ciblée, semi‐quantitative, simultanée d'un nombre élevé de protéines. Dans cette étude, nous utilisons la protéomique pour caractériser et comparer les différences associées à l’âge entre une peau photoexposée et une peau photoprotégée, avec une attention particulière sur l’épiderme, la jonction dermo–épidermique et le derme papillaire. Méthodes Des biopsies de peau de la fesse (photoprotégée) et de l'avant‐bras (photoexposée) de volontaires sains (âgés de 18 à 30 ans ou de ≥ 65 ans) ont été sectionnées transversalement depuis la couche cornée jusqu’à une profondeur de 250 μm. Suite à une électrophorèse SD