Hydroxyapatite/Collagen Three-Dimensional Printed Scaffolds and Their Osteogenic Effects on Human Bone Marrow-Derived Mesenchymal Stem Cells

Three-dimensional (3D) printing provides a novel approach to repair bone defects using customized biomimetic tissue scaffolds. To make a bone substitute closest to natural bone structure and composition, two different types of hydroxyapatite, Nano hydroxyapatite (nHA) and deproteinized bovine bone (...

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Bibliographic Details
Published in:Tissue engineering. Part A 2019-09, Vol.25 (17-18), p.1261-1271
Main Authors: Li, Qing, Lei, Xiongxin, Wang, Xiaofei, Cai, Zhigang, Lyu, Peijun, Zhang, Guifeng
Format: Article
Language:English
Subjects:
3-D printers
Alkaline phosphatase
Alveolar bone
Biocompatibility
Bone biomaterials
Bone composition
Bone growth
Bone loss
Bone marrow
Cbfa-1 protein
Collagen
Collagen (type I)
Crystals
Dentistry
Hydroxyapatite
Mesenchymal stem cells
Mesenchyme
Original Articles
Osteogenesis
Phalloidin
Polymerase chain reaction
Porosity
Printing
Regeneration
Sox9 protein
Stem cells
Trauma
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Summary:Three-dimensional (3D) printing provides a novel approach to repair bone defects using customized biomimetic tissue scaffolds. To make a bone substitute closest to natural bone structure and composition, two different types of hydroxyapatite, Nano hydroxyapatite (nHA) and deproteinized bovine bone (DBB), were dispersed into collagen (CoL) to prepare the bioink for 3D printing. In doing so, a porous architecture was manufactured with 3D printing technology. The physical and chemical properties of the materials were evaluated, including biocompatibility and effect on the osteogenic differentiation of the human bone marrow-derived mesenchymal stem cells (hBMSCs). The XPS, XRD, FTIR, and the mechanical analysis of the material indicated that the two HA were consistent in their elements, but different in their chemical bonds and crystal phases. The SEM results showed the different surface morphologies of the HA crystals as well as the scaffolds, which would be the main factors affecting the internal porous structure of the scaffold. There were no differences between the two composite scaffolds in cell proliferations. FITC-phalloidin/vinculin/DAPI staining indicated that hBMSCs can adhere well to the 3D-printed surfaces. Alkaline phosphatase (ALP) staining reflected ALP expressed on both of the osteogenic-induction medium (OM) group, but not on proliferation medium (PM) group. The real-time polymerase chain reaction results showed the expression levels of osteogenesis-related genes RUNX2, SOX9, OCN, and COL1A1 in OM group were significantly increased after 7 days compared with the PM group ( p  
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2018.0201