Bisphenol S increases EZRIN expression and the detrimental effects induced by dehydroepiandrosterone in rat endometrium

• Published in: Molecular and cellular endocrinology 2019-03, Vol.483, p.64-73
• Main Authors: Demacopulo, Brenda, Kreimann, Erica L.
• Format: Article
• Language: English
• Subjects: BPS; EZRIN; PCOS; POLARITY; Proliferation
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/j.mce.2019.01.006

The use of Bisphenol S (BPS) was proposed as an alternative to Bisphenol A (BPA), a chemical employed in the production of polycarbonate plastics and epoxy resins. BPA is a xenoestrogen that affects normal physiology in several species. It was reported that BPS may also act as a xenoestrogen with harmful effects in the reproductive system. Here we studied the effects of BPS during the induction of a polycystic ovarian syndrome (PCOS)-like condition in rats. Animals were injected daily with vehicle, DHEA 60 mg/kg, BPS 1 μg/kg and DHEA-BPS, for 20 days. Cell apoptosis, cell proliferation, and EZRIN expression were analyzed by immunohistochemistry. We found an increase in PCNA expression, which correlates with cytoplasmic accumulation of the polarization marker, EZRIN, in the BPS treated groups. Additionally, the administration of BPS in the DHEA treated group augmented the stratification and number of “intraepithelial lumina” in the endometrial surface epithelium. •DHEA induces the formation of lumens in the endometrial luminal epithelium.•BPS increases the effects of DHEA in the endometrial epithelium.•BPS increases EZRIN expression in the endometrial and glandular endometrial cells.