First report of New Delhi metallo-β-lactamase-6 (NDM-6) among Klebsiella pneumoniae ST147 strains isolated from dialysis patients in Iran

• Published in: Infection, genetics and evolution genetics and evolution, 2019-04, Vol.69, p.142-145
• Main Authors: Bahramian, Aghil, Shariati, Aref, Azimi, Taher, Sharahi, Javad Yasbolaghi, Bostanghadiri, Narjess, Gachkar, Latif, Ghalavand, Zohreh, Chirani, Alireza Salimi, Erfanimanesh, Soroor, Hashemi, Ali
• Format: Article
• Language: English
• Subjects: Adult; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Bacterial Proteins - genetics; beta-Lactam Resistance; beta-Lactamases - genetics; Carbapenemase; Female; Humans; Iran - epidemiology; Klebsiella Infections - diagnosis; Klebsiella Infections - epidemiology; Klebsiella Infections - etiology; Klebsiella pneumoniae; Klebsiella pneumoniae - classification; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - isolation & purification; Male; Microbial Sensitivity Tests; Middle Aged; Multilocus Sequence Typing; New Delhi metallo- β-lactamase-6; Plasmids - genetics; Renal Dialysis - adverse effects
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2019.01.030

There has been an alarming health-related concern about the growth of New Delhi metallo-β-lactamase. The aims of this study include the phenotypic detection of β-lactamases and molecular characterization of NDM in Klebsiella pneumoniae isolates in Tehran, Iran. A total of 120 K. pneumoniae isolates were collected from hospitalized haemodialysis patients, Tehran, Iran from March 2014 to February 2017. Antibiotic susceptibility tests were conducted using Kirby-Bauer disc diffusion and Broth Microdilution methods according to Clinical and Laboratory Standards Institute guidelines. Metallo-β-lactamase was detected using the Combined Disc Diffusion Test (CDDT), and production of carbapenemase was screened using the Modified Hodge Test. NDM-producing K. pneumoniae strains were screened for the presence of mcr-1 gene, β-lactamase genes, and 16S rRNA methylase genes by Polymerase Chain Reaction and sequencing. Molecular typing of the strains was determined using Repetitive Sequence Based-PCR and Multilocus Sequence Typing. The blaNDM-6 gene was detected in 3 (2.5%) out of 120 isolates from dialysis patients. Also, the three isolates were positive for blaCTX-M-15,blaTEM extended-spectrum β-lactamase genes, armA type plasmid-mediated 16S rRNA methylase and CMY-type plasmid-mediated AmpC β-lactamase. The isolates were identified as MLST sequence type 147 (ST147). This is the first report of blaNDM-6 in K. pneumoniae strains, isolated in Iran. •Of the 120 strains, 86 (71.6%) were identified as ESBL producers.•The blaTEM, blaSHV, and blaCTX-M was detected in 40.69%, 53.4% and 87.20% ESBL-producing isolates, respectively.•Out of 120 isolates, 12 (10%) isolates were found to be carbapenems-resistant•Of 12 isolates, three of them were identified as NDM-6 producing K. pneumoniae.•NDM-6 producing K. pneumoniae isolates were identified as MLST sequence type 147 (ST147).