Advanced approach to activity evaluation for released-active forms of antibodies to interferon-gamma by enzyme-linked immunoassay

Selection of a suitable assay to measure the activity of drugs based on released-active forms (RA forms of Abs) is an important step during their investigation. In this study, ELISA was utilized to examine the effect of RA forms of Abs to interferon gamma on the interaction between monoclonal anti-i...

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Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2019-05, Vol.40 (3), p.250-268
Main Authors: Don, Еlena S., Bobrovnik, Sergey А., Sherriff, Gordon, Myslivets, Andrey А., Tarasov, Sergey А., Epstein, Оleg I.
Format: Article
Language:English
Subjects:
Antibodies
antibodies to interferon-gamma
assay development
Drugs
ELISA
Enzyme-linked immunosorbent assay
Immunoassay
Interferon
interferon gamma
Monoclonal antibodies
released-activity
γ-Interferon
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Summary:Selection of a suitable assay to measure the activity of drugs based on released-active forms (RA forms of Abs) is an important step during their investigation. In this study, ELISA was utilized to examine the effect of RA forms of Abs to interferon gamma on the interaction between monoclonal anti-interferon gamma antibodies and human interferon gamma. The data showed that such RA forms of Abs are able to modulate the antibody interaction with interferon gamma, and it was suggested that the observed influence of RA forms of Abs on 'antibody-antigen' interaction could be used to analyze the activity of this kind of drugs.
ISSN:1532-1819
1532-4230
DOI:10.1080/15321819.2019.1567536