Evaluation of polyherbal ayurvedic formulation ‘Peedantak Vati’ for anti-inflammatory and analgesic properties

• Published in: Journal of ethnopharmacology 2019-05, Vol.235, p.361-374
• Main Authors: Balkrishna, Acharya, Ranjan, Ravikant, Sakat, Sachin S., Sharma, Vinay K., Shukla, Ravikant, Joshi, Khemraj, Devkar, Raviraj, Sharma, Niti, Saklani, Sonia, Pathak, Prateek, Kumari, Pratima, Agarwal, Veena R.
• Format: Article
• Language: English
• Subjects: Analgesic; Edema; HPLC; HPTLC; Inflammation; LC-MS/MS; Nitric oxide; Peedantak Vati; RAW264.7; THP-1
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2019.01.028

Peedantak Vati (PV) is a polyherbal ayurvedic formulation, which is regularly prescribed by the ayurvedic practitioner for the inflammatory disorders and joints pain in India. It is composed of 23 different herbs and minerals, described in ayurvedic text for their anti-inflammatory and analgesic properties. To investigate anti-inflammatory and anti-nociceptive potential of ‘Peedantak Vati’ using in vitro and in vivo methods. In-vitro anti-inflammatory activity of PV was studied by estimating nitric oxide (NO) and LPS-induced pro-inflammatory cytokines IL-6 and TNF-α, using murine macrophage RAW264.7 and human monocyte THP-1 cell lines. PV's anti-inflammatory potential was studied in vivo using carrageenan-induced rat paw edema model. Similarly, anti-nociceptive property of PV was evaluated using hot plate, tail flick, formalin and writhing tests on CD-1 mice. Phytochemical profiling of hydro-alcoholic extract of PV was done using HPLC and HPTLC techniques to identify different marker compounds. These identified marker compounds were confirmed using LC-MS/MS analysis. In vitro results strongly suggest that, PV significantly (p