Composition and cardioprotective effects of Primula veris L. solid herbal extract in experimental chronic heart failure

• Published in: Phytomedicine (Stuttgart) 2019-02, Vol.54, p.17-26
• Main Authors: Latypova, G.M., Bychenkova, M.A., Katayev, V.A., Perfilova, V.N., Tyurenkov, I.N., Mokrousov, I.S., Prokofiev, I.I., Salikhov, Sh.M., Iksanova, G.R.
• Format: Article
• Language: English
• Subjects: Animals; Cardioprotective effect; Cardiotonic Agents - chemistry; Cardiotonic Agents - pharmacology; Chromatography, High Pressure Liquid; Chronic Disease; Disease Models, Animal; Flavonoids; Flavonoids - chemistry; Flavonoids - pharmacology; Heart Failure - chemically induced; Heart Failure - drug therapy; Isoproterenol; Magnetic Resonance Spectroscopy; Male; Myocardial Contraction - drug effects; Myocardium - pathology; Plant Extracts - analysis; Plant Extracts - chemistry; Plant Extracts - pharmacology; Primula - chemistry; Primula veris L; Rats, Wistar; Spectrophotometry, Ultraviolet
• ISSN: 0944-7113; 1618-095X
• DOI: 10.1016/j.phymed.2018.09.015

High interest in chronic heart failure (CHF) is accounted for by its high incidence, poor prognosis, growing number of hospital admissions due to the heart failure relapse, and inadequate treatment. These facts necessitate a search for new pharmacological agents for the CHF correction. Herbal medicinal products appear to be very promising as they have a noticeable therapeutic effect and tend to be more harmless in comparison to the most of synthesized medications. Our aim was to study the composition of the Primula veris L. solid herbal extract (PVSHE) and its effects on the myocardial contractile function in animals with experimental CHF. The study design involved the identification of the raw material composition of the P. veris L. extract. For the experimental part of our research, we used the model of CHF to elucidate the cardioprotective properties of PVSHE. The active extract constituents were isolated by thin-layer chromatography and column chromatography; the extract components were identified by high-performance liquid chromatography, ultraviolet spectroscopy (UVS), and nuclear magnetic resonance spectroscopy (NMRS). To model CHF, L-isoproterenol at a dose of 2.5 mg/kg was intraperitoneally injected to the experimental rats twice a day for 21 days. Cardiac output was assessed with the loading test, adrenoreactivity test, and maximum isometric loading test; CHF markers adrenomedullin and copeptin were detected in blood plasma with ELISA kit for adrenomedullin and copeptin (Coud-Clone Corp., USA). P. veris L. solid herbal extract contains flavonoid aglycons (apigenin, quercetine, kaemferol), flavonoid glycosides (cinarozid, rutin, hyperozid), as well as polymethoxylated flavonoids acting as chemotaxonomic markers for the genus Primula (8-methoxy-flavone; 3′,4′methylenedioxy-5′-methoxyflavone). The substance 3′,4′methylenedioxy-5′-methoxyflavone has been isolated from the primrose herb for the first time. We showed that the PVSHE has a cardioprotective effect when it was administered at a dose of 30 mg/kg in the experimental CHF, as evidenced by a lower number of animal death, lower level of CHF markers in the blood plasma of the experimental animals, the higher increase in rate of myocardial contraction and relaxation, the higher level of left ventricular pressure (LVP) and of maximum intensity of structural performance (MISP), as compared to the control group. P. veris L. solid herbal extract contains flavonoid aglycons, flavonoid glycosides, and p