An easily-performed high-throughput method for plant genomic DNA extraction

Genomic DNA isolation is a crucial technique for researchers studying plant molecular biology. A current widely-used protocol for DNA extraction needs a pestle and mortal for each sample and consumes a large amount of liquid nitrogen in grinding the samples. Most high-throughput methods depend on ex...

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Bibliographic Details
Published in:Analytical biochemistry 2019-03, Vol.569, p.28-30
Main Authors: Yu, Deshui, Zhang, Ju, Tan, Guangxuan, Yu, Ningshu, Wang, Qiuyue, Duan, Qiqi, Qi, Xin, Cheng, Mingjiao, Yan, Chunxue, Wei, Zhangkun, Yu, Zhenmiao, Huang, Wenchao, Li, Chengwei
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
DNA, Plant - analysis
DNA, Plant - isolation & purification
Electrophoresis, Agar Gel
Genome, Plant
High-Throughput Screening Assays - methods
Liquid-Liquid Extraction
Plants - genetics
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Summary:Genomic DNA isolation is a crucial technique for researchers studying plant molecular biology. A current widely-used protocol for DNA extraction needs a pestle and mortal for each sample and consumes a large amount of liquid nitrogen in grinding the samples. Most high-throughput methods depend on expensive machines for tissue homogenization. Here we developed a CTAB-based DNA extraction method using 2.0 mL microcentrifuge tubes for sample processing. This protocol has the advantages that it is suitable for a variety of plants, easily-performed without special equipment, and high-throughput; it effectively avoids sample cross-contamination, and is inexpensive, rapid and safe. •Our protocol with rapid (~100 min), inexpensive, and high-throughput manners for plant genomic DNA isolation.•Using 2 mL microcentrifuge tubes for sample harvest and grinding effectively avoids sample cross-contamination.•This protocol is suitable for a variety of plants.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2019.01.007