Enhancing Luminol Electrochemiluminescence by Combined Use of Cobalt-Based Metal Organic Frameworks and Silver Nanoparticles and Its Application in Ultrasensitive Detection of Cardiac Troponin I

Electrochemiluminescence (ECL) has emerged as one of the most important methods for in vitro diagnosis and detection, but it is still limited in sensitivity for ultrasensitive biodetections. Fast and ultrasensitive detection of biomolecules is critical, especially for the clinical detection of cardi...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-02, Vol.91 (4), p.3048-3054
Main Authors: Wang, Shanshan, Zhao, Yangyang, Wang, Minmin, Li, Haijuan, Saqib, Muhammad, Ge, Chunhua, Zhang, Xiangdong, Jin, Yongdong
Format: Article
Language:English
Subjects:
Biomolecules
Calcium-binding protein
Carbon dioxide
Chemistry
Cobalt
Diagnosis
Electrochemiluminescence
Heart
Immunosensors
In vitro methods and tests
Metal-organic frameworks
Metals
Myocardial infarction
Nanoparticles
Silver
Troponin
Troponin I
Zeolites
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Summary:Electrochemiluminescence (ECL) has emerged as one of the most important methods for in vitro diagnosis and detection, but it is still limited in sensitivity for ultrasensitive biodetections. Fast and ultrasensitive detection of biomolecules is critical, especially for the clinical detection of cardiac troponin I (cTnI) for cardiac infarction diagnosis. In this study, an effective tactic was developed to enhance ECL efficiency of the luminol system, by combined use of Co2+-based metal organic frameworks (MOF), zeolitic imidazolate frameworks (ZIF-67), and luminol-capped Ag nanoparticles (luminol-AgNPs). The integration leads to a pronounced ∼115-fold enhancement in luminol ECL. On the basis of this fascinating sensing platform, a robust label-free ECL immunosensor was constructed for ultrasensitive detection of cTnI, the main marker of myocardial infarction, with good stability and a detection limit as low as 0.58 fg mL–1 (S/N = 3).
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.8b05443