Endless variety for bovine virus diarrhea viruses: new members of a novel subgroup into Pestivirus A from Turkey

• Published in: Tropical animal health and production 2019-06, Vol.51 (5), p.1083-1087
• Main Authors: Oğuzoğlu, Tuba Çiğdem, Koç, Bahattin Taylan, Coşkun, Nüvit, Doğan, Fırat, Duran-Yelken, Selda
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Bovine diarrhea-mucosal disease; Bovine Virus Diarrhea-Mucosal Disease - virology; Breeding; Calves; Cattle; Cellulitis; Congenital defects; Dairy industry; Diarrhea; Diarrhea Virus 1, Bovine Viral - classification; Diarrhea Virus 1, Bovine Viral - genetics; Diarrhea Virus 1, Bovine Viral - isolation & purification; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Female; Life Sciences; Livestock; Livestock industry; Phylogeny; Polymerase chain reaction; Primers; Regular Articles; Reverse transcription; Sequence Analysis, RNA - veterinary; Sequences; Subgroups; Turkey; Veterinary Medicine/Veterinary Science; Viruses; Zoology
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-018-01787-w

As ubiquitous pathogens, bovine virus diarrhea viruses (BVDVs) in cattle have been reported several times in Turkey. Over time, the frequency and importance of this infection has increased for the livestock industries. A total of 1291 animals were sampled from a dairy herd in Turkey suspected of BVDV clinical signs, for instance, reproductive failures (abortion, congenital malformations in calves, repeat breeding, etc.) and interdigital phlegmon in adult animals. Reverse transcription polymerase chain reactions (RT-PCRs) were made by using targeted 5′ untranslated region (UTR), N pro , E2, and NS2-3 pestiviral gene region primers for antigen ELISA-positive samples ( n  = 20). The obtained amplicons were sequenced. Sequence results showed the presence of a new subgroup in Pestivirus A species. This paper describes the nucleotide sequences of a new BVDV 1 (BVDV 1-v) subgroup member.