Ultrasensitive Photoelectrochemical Detection of Multiple Metal Ions Based on Wavelength-Resolved Dual-Signal Output Triggered by Click Reaction

In this work, a click reaction-triggered wavelength-resolved dual-signal output photoelectrochemical (PEC) biosensor with DNAzymes-assisted cleavage recycling amplification was proposed for sensitive triplex metal ions assay. Substantial DNA fragments azido-S1 and azido-S2, derived from the Pb2+ (ta...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-02, Vol.91 (4), p.2861-2868
Main Authors: Deng, Han-Mei, Huang, Liao-Jing, Chai, Ya-Qin, Yuan, Ruo, Yuan, Ya-Li
Format: Article
Language:English
Subjects:
Amplification
Ascorbic acid
Biosensors
Chemical reactions
Chemistry
Cleavage
Copper
Deoxyribonucleic acid
Detection limits
DNA
Immobilization
Lead
Magnesium
Metal ions
Nanomaterials
Nanotechnology
Tumor markers
Wavelength
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Summary:In this work, a click reaction-triggered wavelength-resolved dual-signal output photoelectrochemical (PEC) biosensor with DNAzymes-assisted cleavage recycling amplification was proposed for sensitive triplex metal ions assay. Substantial DNA fragments azido-S1 and azido-S2, derived from the Pb2+ (target 1) and Mg2+ (target 2) dependent cleavage cycle of DNAzymes, respectively, were grafted efficiently on the same alkynyl-DNA (capture DNA) modified electrode via the Cu2+ (target 3) and ascorbic acid (AA) cocatalyzed click reaction, which thus could be subsequently used for immobilization of two different photoactive nanomaterials labeled with single DNA to generate distinguishing dual-signal output for simultaneously sensitive detection of Pb2+ and Mg2+. Furthermore, the control variable method was used for detecting Cu2+ by altering the concentration of Cu2+ in the click reaction. Owing to the usage of the click reaction and target-converted signal amplifying strategy, the utilization rate of cycle output DNAs was largely increased, significantly improving the detection sensitivity of the proposed approach. As a result, low detection limits down to picomolar were acquired for the detection of Pb2+, Mg2+, and Cu2+, providing a versatile, efficient, and sensitive PEC method for multiple assays of various targets such as metal ions, small molecules, and tumor markers.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.8b04831