Ultrasensitive Photoelectrochemical Detection of Multiple Metal Ions Based on Wavelength-Resolved Dual-Signal Output Triggered by Click Reaction

In this work, a click reaction-triggered wavelength-resolved dual-signal output photoelectrochemical (PEC) biosensor with DNAzymes-assisted cleavage recycling amplification was proposed for sensitive triplex metal ions assay. Substantial DNA fragments azido-S1 and azido-S2, derived from the Pb2+ (ta...

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Published in:Analytical chemistry (Washington) 2019-02, Vol.91 (4), p.2861-2868
Main Authors: Deng, Han-Mei, Huang, Liao-Jing, Chai, Ya-Qin, Yuan, Ruo, Yuan, Ya-Li
Format: Article
Language:English
Subjects:
Amplification
Ascorbic acid
Biosensors
Chemical reactions
Chemistry
Cleavage
Copper
Deoxyribonucleic acid
Detection limits
DNA
Immobilization
Lead
Magnesium
Metal ions
Nanomaterials
Nanotechnology
Tumor markers
Wavelength
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cited_by cdi_FETCH-LOGICAL-a413t-d18a1661e67f7a75386784670e312a4a0a59b4ffe9c84dbf29c6fba641c188533
cites cdi_FETCH-LOGICAL-a413t-d18a1661e67f7a75386784670e312a4a0a59b4ffe9c84dbf29c6fba641c188533
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container_issue 4
container_start_page 2861
container_title Analytical chemistry (Washington)
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creator Deng, Han-Mei
Huang, Liao-Jing
Chai, Ya-Qin
Yuan, Ruo
Yuan, Ya-Li
description In this work, a click reaction-triggered wavelength-resolved dual-signal output photoelectrochemical (PEC) biosensor with DNAzymes-assisted cleavage recycling amplification was proposed for sensitive triplex metal ions assay. Substantial DNA fragments azido-S1 and azido-S2, derived from the Pb2+ (target 1) and Mg2+ (target 2) dependent cleavage cycle of DNAzymes, respectively, were grafted efficiently on the same alkynyl-DNA (capture DNA) modified electrode via the Cu2+ (target 3) and ascorbic acid (AA) cocatalyzed click reaction, which thus could be subsequently used for immobilization of two different photoactive nanomaterials labeled with single DNA to generate distinguishing dual-signal output for simultaneously sensitive detection of Pb2+ and Mg2+. Furthermore, the control variable method was used for detecting Cu2+ by altering the concentration of Cu2+ in the click reaction. Owing to the usage of the click reaction and target-converted signal amplifying strategy, the utilization rate of cycle output DNAs was largely increased, significantly improving the detection sensitivity of the proposed approach. As a result, low detection limits down to picomolar were acquired for the detection of Pb2+, Mg2+, and Cu2+, providing a versatile, efficient, and sensitive PEC method for multiple assays of various targets such as metal ions, small molecules, and tumor markers.
doi_str_mv 10.1021/acs.analchem.8b04831
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Substantial DNA fragments azido-S1 and azido-S2, derived from the Pb2+ (target 1) and Mg2+ (target 2) dependent cleavage cycle of DNAzymes, respectively, were grafted efficiently on the same alkynyl-DNA (capture DNA) modified electrode via the Cu2+ (target 3) and ascorbic acid (AA) cocatalyzed click reaction, which thus could be subsequently used for immobilization of two different photoactive nanomaterials labeled with single DNA to generate distinguishing dual-signal output for simultaneously sensitive detection of Pb2+ and Mg2+. Furthermore, the control variable method was used for detecting Cu2+ by altering the concentration of Cu2+ in the click reaction. Owing to the usage of the click reaction and target-converted signal amplifying strategy, the utilization rate of cycle output DNAs was largely increased, significantly improving the detection sensitivity of the proposed approach. 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Furthermore, the control variable method was used for detecting Cu2+ by altering the concentration of Cu2+ in the click reaction. Owing to the usage of the click reaction and target-converted signal amplifying strategy, the utilization rate of cycle output DNAs was largely increased, significantly improving the detection sensitivity of the proposed approach. As a result, low detection limits down to picomolar were acquired for the detection of Pb2+, Mg2+, and Cu2+, providing a versatile, efficient, and sensitive PEC method for multiple assays of various targets such as metal ions, small molecules, and tumor markers.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>30672279</pmid><doi>10.1021/acs.analchem.8b04831</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-4392-9592</orcidid><orcidid>https://orcid.org/0000-0003-3664-6236</orcidid></addata></record>
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Amplification
Ascorbic acid
Biosensors
Chemical reactions
Chemistry
Cleavage
Copper
Deoxyribonucleic acid
Detection limits
DNA
Immobilization
Lead
Magnesium
Metal ions
Nanomaterials
Nanotechnology
Tumor markers
Wavelength
title Ultrasensitive Photoelectrochemical Detection of Multiple Metal Ions Based on Wavelength-Resolved Dual-Signal Output Triggered by Click Reaction
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