Ileal transcriptome analysis in obese rats induced by high-fat diets and an adenoviral infection

• Published in: International Journal of Obesity 2019-11, Vol.43 (11), p.2134-2142
• Main Authors: Sang, Yongming, Shields, Lauren E., Sang, Eric R., Si, Haijun, Pigg, Alexis, Blecha, Frank
• Format: Article
• Language: English
• Subjects: 13/106; 13/51; 14; 14/63; 38; 38/1; 38/77; 38/90; 631/208/135; 631/250/249/2510; 64; Adipose tissue; Analysis; Body weight; Causes of; Circadian rhythm; Circadian rhythms; Diet; Epidemics; Epidemiology; Gene expression; Genes; Genetic aspects; Genomes; Health Promotion and Disease Prevention; High fat diet; Ileum; Immunity; Interferon; Internal Medicine; Intestine; Medicine; Medicine & Public Health; Metabolic Diseases; Obesity; Phenotypes; Public Health; Ribonucleic acid; RNA; Rodents; Synergistic effect; Viruses
• ISSN: 0307-0565; 1476-5497
• DOI: 10.1038/s41366-019-0323-2

Background Obesity has become a worldwide epidemic affecting millions of people. Obesity and associated health consequences tend to be complicated by diverse causes and multi-systemic involvement. Previous studies have investigated obesity induced by a single factor, such as a high-fat diet (HF) of typical energy-dense food and infection by an adipogenic virus, such as a widely studied human adenovirus serotype 36 (Ad-36). In this study, we hypothesized and investigated the synergistic effect of two causal factors, HF and Ad-36, in obesity induction. Methods The 7-week-old Wistar rats ( n  = 1214/group) were randomly divided into weight-matched groups and induced for obesity with mock-control, HF, Ad-36, or HF + Ad-36 for 8–30 weeks, and compared for obesity phenotype. A global transcriptomic RNA-Seq analysis was used to profile signature gene response pathways in ileal tissues from 8-week control and obese animals during this early phase of obesity induction. Results HF only and particularly co-administration of Ad-36 and HF (HF + Ad-36) induced significant obesity in rats ( p