Haplotype-phased genome and evolution of phytonutrient pathways of tetraploid blueberry

• Published in: Gigascience 2019-03, Vol.8 (3)
• Main Authors: Colle, Marivi, Leisner, Courtney P, Wai, Ching Man, Ou, Shujun, Bird, Kevin A, Wang, Jie, Wisecaver, Jennifer H, Yocca, Alan E, Alger, Elizabeth I, Tang, Haibao, Xiong, Zhiyong, Callow, Pete, Ben-Zvi, Gil, Brodt, Avital, Baruch, Kobi, Swale, Thomas, Shiue, Lily, Song, Guo-qing, Childs, Kevin L, Schilmiller, Anthony, Vorsa, Nicholi, Buell, C Robin, VanBuren, Robert, Jiang, Ning, Edger, Patrick P
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Biosynthesis; Biosynthetic Pathways - genetics; Blueberries; Blueberry Plants - genetics; Chromosomes; Chromosomes, Plant - genetics; Cultivars; Draper protein; Evolution, Molecular; Food quality; Fruit - genetics; Fruit - growth & development; Fruits; Gene Duplication; Gene expression; Gene Expression Regulation, Plant; Genes, Plant; Genome, Plant; Genomes; Haplotypes; Haplotypes - genetics; Metabolites; Molecular Sequence Annotation; Multigene Family; Nutrients; Nutrition; Phytochemicals; Phytochemicals - chemistry; Phytochemicals - genetics; Plant breeding; Polyploidy; RNA, Messenger - genetics; RNA, Messenger - metabolism; Tetraploidy; Vaccinium corymbosum
• ISSN: 2047-217X; 2047-217X
• DOI: 10.1093/gigascience/giz012

Abstract Background Highbush blueberry (Vaccinium corymbosum) has long been consumed for its unique flavor and composition of health-promoting phytonutrients. However, breeding efforts to improve fruit quality in blueberry have been greatly hampered by the lack of adequate genomic resources and a limited understanding of the underlying genetics encoding key traits. The genome of highbush blueberry has been particularly challenging to assemble due, in large part, to its polyploid nature and genome size. Findings Here, we present a chromosome-scale and haplotype-phased genome assembly of the cultivar “Draper,” which has the highest antioxidant levels among a diversity panel of 71 cultivars and 13 wild Vaccinium species. We leveraged this genome, combined with gene expression and metabolite data measured across fruit development, to identify candidate genes involved in the biosynthesis of important phytonutrients among other metabolites associated with superior fruit quality. Genome-wide analyses revealed that both polyploidy and tandem gene duplications modified various pathways involved in the biosynthesis of key phytonutrients. Furthermore, gene expression analyses hint at the presence of a spatial-temporal specific dominantly expressed subgenome including during fruit development. Conclusions These findings and the reference genome will serve as a valuable resource to guide future genome-enabled breeding of important agronomic traits in highbush blueberry.