Isolation and characterization of CAC antenna proteins and photosystem I supercomplex from the cryptophytic alga Rhodomonas salina

In the present paper, we report an improved method combining sucrose density gradient with ion‐exchange chromatography for the isolation of pure chlorophyll a/c antenna proteins from the model cryptophytic alga Rhodomonas salina. Antennas were used for in vitro quenching experiments in the absence o...

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Bibliographic Details
Published in:Physiologia plantarum 2019-05, Vol.166 (1), p.309-319
Main Authors: Kuthanová Trsková, Eliška, Bína, David, Santabarbara, Stefano, Sobotka, Roman, Kaňa, Radek, Belgio, Erica
Format: Article
Language:English
Subjects:
Algae
Antennas
Chlorophyll
Fluorescence
Fluorescence spectroscopy
Photochemicals
Photosystem I
Protein interaction
Proteins
Quenching
Rhodomonas
Sucrose
Sugar
Xanthophylls
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Summary:In the present paper, we report an improved method combining sucrose density gradient with ion‐exchange chromatography for the isolation of pure chlorophyll a/c antenna proteins from the model cryptophytic alga Rhodomonas salina. Antennas were used for in vitro quenching experiments in the absence of xanthophylls, showing that protein aggregation is a plausible mechanism behind non‐photochemical quenching in R. salina. From sucrose gradient, it was also possible to purify a functional photosystem I supercomplex, which was in turn characterized by steady‐state and time‐resolved fluorescence spectroscopy. R. salina photosystem I showed a remarkably fast photochemical trapping rate, similar to what recently reported for other red clade algae such as Chromera velia and Phaeodactylum tricornutum. The method reported therefore may also be suitable for other still partially unexplored algae, such as cryptophytes.
ISSN:0031-9317
1399-3054
DOI:10.1111/ppl.12928