Low Expression of Toll-like Receptor 4 Is Associated With Poor Prognosis in Bladder Cancer

The aim of this study was to elucidate the relationship between the progression of bladder cancer (BCa) and TLR4 expression. The relationship between TLR4 expression and prognosis of BCa patients was analyzed using a publicly available database and immunohistochemical staining of clinical samples. T...

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Bibliographic Details
Published in:Anticancer research 2019-02, Vol.39 (2), p.703-711
Main Authors: Kusuhara, Yoshito, Daizumoto, Kei, Kawai, Kaichi, Hirayama, Kento, Kowada, Minoru, Shintani, Terumichi, Fukuhara, Yayoi, Dondoo, Tsogt-Ochir, Ozaki, Keisuke, Tsuda, Megumi, Fukawa, Tomoya, Nakatsuji, Hiroyoshi, Bando, Yoshimi, Uehara, Hisanori, Fukumori, Tomoharu, Takahashi, Masayuki, Kanayama, Hiro-Omi
Format: Article
Language:English
Subjects:
Aged
Biomarkers, Tumor - metabolism
Bladder
Bladder cancer
Cancer
Cell Differentiation
Cell Line, Tumor
Computational Biology
Depletion
Differentiation
Disease Progression
DNA microarrays
Female
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Invasiveness
Male
Middle Aged
Neoplasm Invasiveness
Oligonucleotide Array Sequence Analysis
Prognosis
RNA, Small Interfering - metabolism
TLR4 protein
Toll-Like Receptor 4 - metabolism
Toll-like receptors
Urinary Bladder Neoplasms - diagnosis
Urinary Bladder Neoplasms - metabolism
Urinary Bladder Neoplasms - pathology
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Summary:The aim of this study was to elucidate the relationship between the progression of bladder cancer (BCa) and TLR4 expression. The relationship between TLR4 expression and prognosis of BCa patients was analyzed using a publicly available database and immunohistochemical staining of clinical samples. The effect of TLR4 knockdown was also examined on the invasive capabilities of BCa cells. Finally, to investigate the biological function of TLR4, the gene expression profile of TLR4-depleted BCa cells was analyzed by microarray analysis. Expression of TLR4 was inversely associated with prognosis of patients with invasive BCa, and depletion of TLR4 significantly enhanced the invasive capability of BCa cells. Gene expression profiling revealed that depletion of TLR4 led to high expression of epithelial differentiation genes. Furthermore, expression of TLR4 was found to be extremely low in areas of squamous differentiation. Low TLR4 expression was correlated with tumor progression.
ISSN:0250-7005
1791-7530
DOI:10.21873/anticanres.13166