In situ bioremediation of hexavalent chromium in presence of iron by dried sludge bacteria exposed to high chromium concentration

• Published in: Ecotoxicology and environmental safety 2019-05, Vol.172, p.281-289
• Main Authors: Bansal, Neetu, Coetzee, Johan J., Chirwa, Evans M.N.
• Format: Article
• Language: English
• Subjects: Bacteria - metabolism; Biocatalysis; Biodegradation, Environmental; Chromium - metabolism; Chromium reducing bacteria; Chromium stabilisation; Ferrochrome slag; Hydrogen-Ion Concentration; In situ bioremediation; Industrial Waste; Inhibition kinetics; Iron - chemistry; Kinetics; Oxidation-Reduction; Sewage - microbiology
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2019.01.094

Stability of chromium in the ferrochrome slag dumps and leachate are affected by pH, redox potential and the presence of other metallic species in the slag. It is desirable to keep chromium in slag dumps in the trivalent [Cr(III)] state because trivalent chromium is 1000 times less toxic to living organisms than the hexavalent form [Cr(VI)]. Due to the low toxicity and low mobility of Cr(III), it is recommended to convert Cr(VI) to Cr(III) wherever possible to protect the health of living organisms. In this study, the role of Cr(VI) reducing organisms for stabilising chromium in slag dumps was evaluated in the presence of iron [oxidation states Fe(II) and Fe(III)]. The study showed that stabilisation of chromium species in the trivalent state was most favourable under aerated conditions. Up to 100 mg/L Cr(VI) was reduced in less than 24 h by cultures grown under aerobic conditions in the presence of Fe(III). A much shorter time (6 h) was required to reduce the same amount of Cr(VI) in the presence of Fe(II). When oxygen was completely excluded, it was only possible to reduce 20 mg/L in about 48 h which was much slower than the removal of 100 mg/L in less than 24 h under aerated conditions. Fe(II) contributed directly to catalytic reduction of Cr(VI) reduction whereas Fe(III) was beneficial to Cr(VI) reduction up to an initial Cr(VI) concentration of 75 mg/L. Evaluation of Cr(VI) reduction kinetics showed that Cr(VI) reduction under aerobic conditions followed the non-competitively inhibited mixed-order reaction. Cr(VI) reduction in sealed reactor vessels, under anaerobic conditions, followed a modified non-competitive inhibition reaction model. The results indicate that chromium stabilisation in ferrochrome slag dumps would require maintenance of a fully aerated dump supplemented by a culture of Cr(VI) reducing organisms. •Effect of Fe (II)and Fe(III) on bioremediation of Cr(VI).•Aerobic reduction rate was higher than anaerobic reduction rate.•In situ bioremediation of Cr(VI) can be an effective remediation strategy for the ferrochrome slag dumps and leachate.