Variation in Glycogen Distribution among Freshwater Bivalve Tissues: Simplified Protocol and Implications

Glycogen is a primary metabolic reserve in bivalves and can be suitable for the evaluation of bivalve condition and health status, but the use of glycogen as a diagnostic tool in aquaculture and biomonitoring is still relatively rare. A tissue biopsy combined with a simplified phenol–sulfuric acid m...

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Bibliographic Details
Published in:Journal of aquatic animal health 2019-03, Vol.31 (1), p.107-111
Main Authors: Vodáková, Barbora, Douda, Karel
Format: Article
Language:English
Subjects:
Animals
Anodonta - chemistry
Ecotoxicology - instrumentation
Ecotoxicology - methods
Energy Metabolism
Glycogen - analysis
Organ Specificity
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Summary:Glycogen is a primary metabolic reserve in bivalves and can be suitable for the evaluation of bivalve condition and health status, but the use of glycogen as a diagnostic tool in aquaculture and biomonitoring is still relatively rare. A tissue biopsy combined with a simplified phenol–sulfuric acid method was used in this study to evaluate the inter‐ and intraindividual variation in the glycogen concentrations among several tissues (foot, mantle, gills, adductor muscle) of the unionid bivalve, the duck mussel Anodonta anatina. This short report documents that individual bivalves differ in the spatial distribution of glycogen among tissues. Sampling of different types of tissues can cause distinct results in the evaluation of energetic reserves at the individual level. At the same time, spatial variability in glycogen content has the potential to provide a more detailed evaluation of physiological conditions based on tissue‐specific glycogen storage. The results obtained and the simplified methodology provide a new opportunity for researching the energetic reserves and health status of freshwater mussels in various applications.
ISSN:0899-7659
1548-8667
DOI:10.1002/aah.10057