Chronic and oxidative stress association with total count of endothelial microvesicles in healthy young male plasma

• Published in: Advances in clinical and experimental medicine : official organ Wroclaw Medical University 2019-05, Vol.28 (5), p.683-692
• Main Authors: Žėkas, Vytautas, Matuzevičienė, Reda, Karčiauskaitė, Dovilė, Mažeikienė, Asta, Burokienė, Neringa, Radzevičius, Mantas, Janilionienė, Aušra, Linkevičiūtė, Aušra, Kučinskienė, Zita Aušrelė
• Format: Article
• Language: English
• Subjects: Adult; Biomarkers; Cell Movement - physiology; Cell-Derived Microparticles - physiology; Endothelial Cells - physiology; Endothelium; Healthy Volunteers; Humans; Male; Middle Aged; Oxidative Stress
• ISSN: 1899-5276
• DOI: 10.17219/acem/94144

Chronic and oxidative stress promotes injury to the endothelium. This happens early in the disease and novel biomarkers describing the rate of the damage may be important in early diagnostics and prevention. Microvesicles are shed from endothelial cells in response to oxidative stress, inflammation, coagulation, and angiogenesis. Their increased level in plasma could reflect the state of the endothelium. The objective of this study was to test the association between oxidative and chronic stress markers, atherosclerosis risk factors and endothelial microvesicle (EMV) count in peripheral blood. The study included 81 males, aged 25-55 years and apparently healthy. Venous blood samples were labeled with anti-CD144-FITC, anti-CD105-BV421, anti-CD42a-PerCP, anti-CD62e-PE, anti-CD31-APCy7, and anti-CD61-APC (BD Biosciences, San Jose, USA), and tested using a BD LSR Fortessa cytometer (BD Biosciences). Events were gated on forward and side-scattered light parameters. Malondialdehyde (MDA) and cortisol concentrations were measured using high-performance liquid chromatography (HPLC). Four populations of EMV expressing a combination of CD105+, CD31+, CD144+, and CD62e with CD42aor CD42a+ markers were examined. We found correlations between MDA concentration and hair cortisol and a total count of CD144+ microvesicles, and weak correlations with diastolic blood pressure (DBP) (p = 0.003, r = 0.324) and systolic blood pressure (SBP) (p = 0.016, r = 0.267), especially with the microvesicles carrying CD62e. There was a median difference of CD105+ microvesicle count between smoking (n = 13) and non-smoking (n = 68) individuals. A predictive model showed an association between CD144+ microvesicle counts with cortisol and MDA concentrations and waist circumference. In conclusion, our data and predictive model showed that the total counts of microvesicle populations were associated with stress-related parameters - cortisol and MDA concentrations; expression of CD62e in various populations of EMV and the ratio of CD144+ to CD105+/CD62e+ were associated with increased DBP and SBP, and also with total cholesterol concentration in healthy young male population.