Proteomic analysis of the bio-corona formed on the surface of (Au, Ag, Pt)-nanoparticles in human serum

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2019-05, Vol.177, p.141-148
• Main Authors: del Pilar Chantada-Vázquez, María, López, Antonio Castro, Bravo, Susana B., Vázquez-Estévez, Sergio, Acea-Nebril, Benigno, Núñez, Cristina
• Format: Article
• Language: English
• Subjects: Gold nanoparticles (AuNPs); Human serum; Platinum nanoparticles (PtNPs); Prognostic biomarker; Protein corona; Silver nanoparticles (AgNPs)
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2019.01.056

[Display omitted] •Interaction of AuNPs, AgNPs and PtNPs with human serum formed a protein corona.•The protein corona formation depends on the core material composition and its size.•Proteins with different functionality were detected in all protein coronas. Adsorption of biomolecules onto nanoparticles surface in biological samples led to the formation of a bio-corona, it could modified the “identity” of nanoparticles, contributing to the determination of their toxicity and biocompatibility. Gel electrophoresis in combination with liquid chromatography-tandem mass spectrometry (LC–MS/MS) was employed to qualitatively analyze and identify the human serum proteins adsorbed on the surface of three different nanomaterials stabilized with citrate: 10.02 ± 0.91 nm gold nanoparticles (AuNPs), 9.73 ± 1.70 nm silver nanoparticles (AgNPs) and, 2.40 ± 0.30 nm platinum nanoparticles (PtNPs). An exhaustive analysis and classification of all identified proteins related with their function were also developed.