Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes

• Published in: Theriogenology 2019-04, Vol.128, p.140-148
• Main Authors: Casares-Crespo, Lucía, Fernández-Serrano, Paula, Viudes-de-Castro, María P.
• Format: Article
• Language: English
• Subjects: Genotype; LC-MS/MS; Proteome; Rabbit; Spermatozoa
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2019.01.026

The present study was conducted to characterise rabbit sperm proteins focusing on the influence of the genetic origin. Six samples were recovered during two months from five males from genotype A (New Zealand White origin) and five from genotype R (California origin). Sperm proteins were extracted and subjected to in-gel digestion nano LC-MS/MS and bioinformatics analysis. The resulting library included 487 identified proteins validated with ≥95% Confidence (unused Score ≥ 1.3). All the identified proteins belonged to Oryctolagus cuniculus taxonomy. These data are available via ProteomeXchange with identifier PXD007989. Only 7 proteins were specifically implicated in reproductive processes according to Gene Ontology annotation. Regarding the comparison of the sperm proteins abundance between genotypes, forty proteins were differentially expressed. Among them, 25 proteins were over-expressed in genotype A, while 15 proteins were over-expressed in genotype R. In conclusion, this study characterizes for the first time rabbit sperm proteins and provides evidence that genotype is related to a specific abundance of spermatozoa proteins. [Display omitted] •First in-depth characterization of rabbit sperm proteome.•487 proteins were identified and quantified in rabbit spermatozoa.•Genotype is related to specific proteins abundance in sperm.•A publicly accessible database of the rabbit sperm proteome was created.