PTBP2 exon 10 inclusion is associated with the progression of CML and it is BCR-ABL1 dependent

• Published in: The international journal of biochemistry & cell biology 2019-04, Vol.109, p.69-75
• Main Authors: Nandagopalan, S. Rajashree, Agatheeswaran, Subramaniam, Vadlamudi, Yellamandayya, Biswas, Sutapa, Biswas, Ghanshyam, Pattnayak, Naresh Chandra, Chakraborty, Soumen
• Format: Article
• Language: English
• Subjects: Alternative splicing; BCR-ABL1; CML; Imatinib; PTBP2; RNA binding proteins
• ISSN: 1357-2725; 1878-5875
• DOI: 10.1016/j.biocel.2019.01.018

•PTBP2 expression was found to be up-regulated in CML cell lines and in CML-blast crisis patients.•PTBP2 exon 10 inclusion increases with CML disease progression and it is BCR-ABL1 dependent.•In vitro studies showed that silencing of PTBP2 leads to reduced cell growth, increased G2/M arrest and increased apoptosis of CML cells. Altered or aberrant expression of several splicing factors leads to the progression of different cancers. Though there are several ongoing studies underscoring the role of the splicing regulator polypyrimidine tract binding protein 2 (PTBP2) in neuronal cells, we unveil the role of PTBP2 in chronic myeloid leukemia (CML). Different RNA binding proteins (RBP's) earlier reported in chronic myeloid leukemia blast crisis (CML-BC) cases (n = 28) from Radich Oncomine leukemia dataset, were compared. We observed increased expression of MSI2 followed by PTBP2 in BC cases and increased PTBP2 expression in relapsed cases (n = 10) from the same dataset compared to other RBPs. We also observed increased PTBP2 exon 10 inclusion in KCL22, a granulocytic lineage CML cell line when compared to other CML cell lines of different lineages. As PTBP2 protein expression is associated with PTBP2 exon 10 inclusion, we observed in cell lines and in a set of progressed cases (n = 4) that increased BCR-ABL1 expression potentiates PTBP2 exon 10 inclusion and thus confers the existence of a functional protein. Inhibition of BCR-ABL1 with imatinib not only blocks the inclusion of exon 10 but also deregulates PTBP2 expression in CML cells. Knockdown of PTBP2 in KCL22 cells leads to reduced cell proliferation, increased G2/M cell cycle arrest and increased apoptosis. Taken together our study portrays PTBP2 as a new possible target for CML and progressive inclusion/exclusion of PTBP2 exon 10 might play an important role in CML progression.