Microbial community and fermentation characteristic of Italian ryegrass silage prepared with corn stover and lactic acid bacteria

•This is the first investigation on microbial community of mixed silage using SMRT.•Mixed silage is characterized by high bacterial diversity.•Lactobacillus plantarum was the main species affecting silage fermentation.•Heterofermentative Lactobacillus species produced acetate in the silage.•The SMRT...

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Bibliographic Details
Published in:Bioresource technology 2019-05, Vol.279, p.166-173
Main Authors: Yan, Yanhong, Li, Xiaomei, Guan, Hao, Huang, Linkai, Ma, Xiao, Peng, Yan, Li, Zhou, Nie, Gang, Zhou, Jiqiong, Yang, Wenyu, Cai, Yimin, Zhang, Xinquan
Format: Article
Language:English
Subjects:
Corn stover
Italian ryegrass silage
Lactic acid bacteria
Microbial community
SMRT
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Summary:•This is the first investigation on microbial community of mixed silage using SMRT.•Mixed silage is characterized by high bacterial diversity.•Lactobacillus plantarum was the main species affecting silage fermentation.•Heterofermentative Lactobacillus species produced acetate in the silage.•The SMRT method provides deep insights into the microbial composition of silages. The bacterial community determined via PacBio single molecule, real-time sequencing technology (SMRT) and the fermentation characteristics of Italian ryegrass (IR, 82% moisture) silage prepared with corn stover (CS) were investigated. A selected strain of Lactobacillus plantarum (L694) and a commercial inoculant stain of Lactobacillus plantarum (LP) were used as additives. Lactic acid bacteria (LAB) effectively improved silage quality. After fermentation, Lactobacillus plantarum was the dominant species in IR + LP and IR + L694 treatments, which led to higher (P 
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2019.01.107