Molecular and functional insights into Transient Receptor Potential Vanilloid 1 (TRPV1) in bull spermatozoa

In view of the limited information available on functional significance of TRPV1 in regulating sperm functions, present study was undertaken on bull spermatozoa. Sixty four ejaculates were collected from four Hariana bulls and were used for molecular and functional characterisation of TRPV1. Immunob...

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Bibliographic Details
Published in:Theriogenology 2019-04, Vol.128, p.207-217
Main Authors: Kumar, Akshay, Mishra, Abhishek Kumar, Singh, Vijay, Yadav, Sarvajeet, Saxena, Atul, Garg, Satish Kumar, Swain, Dilip Kumar
Format: Article
Language:English
Subjects:
AEA
Bull
Capacitation
Hyperactive motility
Progressive sperm motility
Spermatozoa
TRPV1
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Summary:In view of the limited information available on functional significance of TRPV1 in regulating sperm functions, present study was undertaken on bull spermatozoa. Sixty four ejaculates were collected from four Hariana bulls and were used for molecular and functional characterisation of TRPV1. Immunoblotting using TRPV1 specific antibody revealed the presence of a single band of 104 kDa corresponding to TRPV1 in Hariana bull spermatozoa. Indirect immuno fluorescence revealed positive immune-reactivity to TRPV1 at acrosomal, pre-acrosomal, post acrosomal and flagellar regions of spermatozoa. Based on the results of pilot study dose-response analysis, doses of anandamide (AEA; 0.3 μM) and capsazepine (Cp; 10 μM) were selected for further studies. Three groups of semen samples (control 100 μL diluted semen having 1 × 106 spermatozoa; anandamide (3 μL AEA+97 μL of diluted semen containing 1 × 106 spermatozoa and Cp (1 μL Cp+99 μL of diluted semen containing 1 × 106 spermatozoa) were used to study the functional involvement of TRPV1 in bull spermatozoa. Blocking of TRPV1 with Cp resulted in significant (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2019.01.029