Stress‐related hormones affect carbohydrate metabolism in Drosophila females

• Published in: Archives of insect biochemistry and physiology 2019-05, Vol.101 (1), p.e21540-n/a
• Main Authors: Karpova, Evgenia K., Eremina, Margarita A., Pirozhkova, Darya S., Gruntenko, Nataly E.
• Format: Article
• Language: English
• Subjects: Animals; Carbohydrate metabolism; Carbohydrate Metabolism - drug effects; Carbohydrates; Dihydroxyphenylalanine - pharmacology; Dopamine; Dopamine - metabolism; Drosophila; Drosophila melanogaster; Drosophila melanogaster - drug effects; Drosophila melanogaster - metabolism; Drosophila melanogaster - physiology; Drosophila Proteins - metabolism; Exposure; Female; Females; Gene Expression; Heat stress; Heat tolerance; Heat-Shock Response - physiology; Hormones; Insects; Insulin; Insulin - metabolism; Insulin - pharmacology; juvenile hormone; Juvenile hormones; Juvenile Hormones - pharmacology; Metabolism; Signal transduction; Signal Transduction - drug effects; Signal Transduction - physiology; Transcription; Trehalase - genetics; Trehalase - metabolism; Trehalose; Trehalose - metabolism
• ISSN: 0739-4462; 1520-6327
• DOI: 10.1002/arch.21540

A highly conservative insulin signaling pathway, stable work of which is indicated by carbohydrates metabolism, is also known to play an important role in the control of stress resistance. Here we demonstrate that exposure to heat stress leads to a rise in the levels of trehalose and glucose in females of Drosophila melanogaster, but does not affect the expression level of the trehalase (Treh) gene. We have shown that the rise in juvenile hormone (JH) and dopamine decreases levels of both carbohydrates under the normal conditions but brings them to values close to normal following the stress exposure. The data obtained suggest that (a) dopamine and JH involved in the neuroendocrine stress reaction in D. melanogaster also take part in the regulation of carbohydrates metabolism, tending to normalize it after stress; (b) the regulation of trehalose content under stress does not occur at the level of transcription of the degrading enzyme. The effect of a change in the level of dopamine (l‐DOPA feeding of flies) on carbohydrates (glucose and trehalose) metabolism in 3‐day‐old Canton S females od D. melanogaster under normal conditions and heat stress (30 min, 38°C). Each value is an average of 8 to 16 measurements (means ± SEM). The asterisk indicated significant differences from l‐DOPA feeding flies and the diamond indicates significant differences between heat treated and control flies of the same group. (Two diamonds or asterisks, to p < 0.01; three diamonds or asterisks, to p < 0.001).