Comparative Serum Proteomic Analysis of the Effects of Sodium Selenate on a Mouse Model of Alzheimer’s Disease

• Published in: Biological trace element research 2019-12, Vol.192 (2), p.263-276
• Main Authors: Chen, Ping, Wang, Li-Xiang, Sui, Xiao-Jing, Li, Shui-Ming, Wang, Yong, Liu, Qiong, Ni, Jia-Zuan
• Format: Article
• Language: English
• Subjects: alpha 1-Antitrypsin - blood; alpha 1-Antitrypsin - metabolism; Alzheimer Disease - blood; Alzheimer Disease - drug therapy; Alzheimer Disease - pathology; Alzheimer's disease; Analytical methods; Animals; Biochemistry; Biomedical and Life Sciences; Biotechnology; Dietary supplements; Disease Models, Animal; Drinking water; Electrophoresis; Fluorescence; Gel electrophoresis; Gels; Glycoproteins; Glycoproteins - antagonists & inhibitors; Glycoproteins - blood; Haptoglobin; Haptoglobins - analysis; Haptoglobins - antagonists & inhibitors; Life Sciences; Lipid metabolism; Lipids; Metabolism; Mice; Mice, Inbred Strains; Mice, Transgenic; Nutrition; Oncology; Pathogenesis; Pathology; Prealbumin - analysis; Prealbumin - antagonists & inhibitors; Pregnancy-Associated alpha 2-Macroglobulins - analysis; Pregnancy-Associated alpha 2-Macroglobulins - antagonists & inhibitors; Proteins; Proteomics; Selenic Acid - pharmacology; Selenium; Serum; Serum proteins; Sodium; Sodium selenate; Trace elements; Trace elements (nutrients); Transgenic mice; Transthyretin
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-019-01676-9

Selenium (Se), as a nutritionally essential trace element, has been shown to decrease with age and is closely related to Alzheimer’s disease (AD). To probe the effects of Se on AD pathology, two-dimensional fluorescence difference gel electrophoresis was applied to the serum samples collected from the wild-type (WT) mice and the triple transgenic (PS1M146V/AβPPSwe/TauP301L) AD mice (3xTg-AD), treated with or without sodium selenate in drinking water for 4 months beginning at 2 months of age. Proteomics results revealed 17 differentially expressed proteins between WT and 3xTg-AD mice. It was found that the administration of selenate reversed the alterations of the differentially expressed serum proteins by up-regulating 13 proteins and down-regulating 2 proteins which were reported to be involved in the key pathogenesis of AD, including regulation of Aβ production, lipid metabolism regulation, and anti-inflammation. These results suggested that a dietary supplement with selenate is effective for prevention and treatment of AD, and the mechanism was maybe related to its role in Aβ regulation, lipid metabolism, and anti-inflammation. Moreover, we also presented that α-2 macroglobulin, transthyretin, haptoglobin, alpha-2-HS-glycoprotein, and alpha-1-antitrypsin in the serum can be used to evaluate the effect of selenate on AD pathology.