Selection and characterization of novel DNA aptamer against colorectal carcinoma Caco‐2 cells

Aptamers are short, single‐stranded nucleic acid (DNA or RNA) oligonucleotides that can be obtained by a technique called systematic evolution of ligands by exponential enrichment (SELEX) in vitro. Due to superior properties such as small size, high binding affinity, and stability, they are consider...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2019-05, Vol.66 (3), p.412-418
Main Authors: Maimaitiyiming, Yasen, Yang, Chang, Wang, Yun, Hussain, Liaqat, Naranmandura, Hua
Format: Article
Language:English
Subjects:
Affinity
Aptamers
Biological evolution
Caco‐2
Cancer
CD133
cell SELEX
Colorectal cancer
Colorectal carcinoma
Confocal microscopy
Deoxyribonucleic acid
DNA
Feasibility studies
Medical treatment
Microscopy
Oligonucleotides
Ribonucleic acid
RNA
ssDNA aptamer
targeted therapy
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Summary:Aptamers are short, single‐stranded nucleic acid (DNA or RNA) oligonucleotides that can be obtained by a technique called systematic evolution of ligands by exponential enrichment (SELEX) in vitro. Due to superior properties such as small size, high binding affinity, and stability, they are considered to be feasible tools for diagnosis and treatment of disease. In the current study, we attempted to screen a high‐affinity DNA aptamer to selectively target the colorectal carcinoma Caco‐2 cells by using cell‐based SELEX approach. After 14 consecutive rounds of selection, aptamer ApC1 was identified. Confocal microscopy results revealed that ApC1 could rapidly internalize into Caco‐2 cells but not HEK 293 cells. Moreover, it showed high specificity to Caco‐2 cells rather than other cell lines such as 293T, HeLa, MCF‐7, HL‐60, and NB4. Collectively, our results demonstrated that aptamer ApC1 has high specificity to colorectal carcinoma Caco‐2 cells, which could be further applied for targeted therapy of colorectal cancer in future studies.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.1737