Characterization of the Biosynthetic Gene Cluster for the Antibiotic Armeniaspirols in Streptomyces armeniacus

Armeniaspirols (1–3) are potent antibiotics against Gram-positive pathogens. Through a biosynthetic investigation, we identified four enzymes involved in the structural modification of 1–3. Manipulation of their activity led to the generation of 4–6 and nine novel analogues, 7–15. Bioactivity assess...

Full description

Saved in:
Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2019-02, Vol.82 (2), p.318-323
Main Authors: Qiao, Yongjian, Yan, Jiayan, Jia, Jia, Xue, Jiao, Qu, Xudong, Hu, Yunfeng, Deng, Zixin, Bi, Hongkai, Zhu, Dongqing
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - biosynthesis
Multigene Family
Pyrroles - metabolism
Pyrroles - pharmacology
Spiro Compounds - metabolism
Spiro Compounds - pharmacology
Streptomyces - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Armeniaspirols (1–3) are potent antibiotics against Gram-positive pathogens. Through a biosynthetic investigation, we identified four enzymes involved in the structural modification of 1–3. Manipulation of their activity led to the generation of 4–6 and nine novel analogues, 7–15. Bioactivity assessments revealed that the pyrrole chloro group and the methyl group are important for the antimicrobial activities of armeniaspirols, which lays the foundation for future structure optimization and mechanism of action studies of armeniaspirols.
ISSN:0163-3864
1520-6025
DOI:10.1021/acs.jnatprod.8b00753