Nanocapsules of Sterculia striata acetylated polysaccharide as a potential monomeric amphotericin B delivery matrix

• Published in: International journal of biological macromolecules 2019-06, Vol.130, p.655-663
• Main Authors: Matoso Sombra, Fernanda, Richter, Ana Rosa, de Araújo, Alyne Rodrigues, de Oliveira Silva Ribeiro, Fábio, de Fátima Souza Mendes, Josilayne, dos Santos Fontenelle, Raquel Oliveira, da Silva, Durcilene Alves, Beserra de Paula, Haroldo César, Pessoa de Andrade Feitosa, Judith, Martín Goycoolea, Francisco, Monteiro de Paula, Regina Célia
• Format: Article
• Language: English
• Subjects: Acetylated derivatives; Amphotericin B; Nanocapsules; Sterculia striata
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.02.076

Stable oil nanocapsules based on acetylated Sterculia striata polysaccharide (ASSP) were produced without the use of a surfactant, and derivatives of ASSP with four different degrees of substitution (DS) were synthesised. The data revealed that only derivatives with high DS were able to produce nanocapsules (NC), which exhibited monomodal size distribution profiles with a Z-average particle size, ζ-potential, and polydispersity index (PDI) that were dependent on ASSP DS and concentration. Nanocapsules were loaded with amphotericin B (AMB) with encapsulation efficiencies (EE%) that were dependent on drug and ASSP concentrations and DS. A maximum EE% value of 99.2% was achieved, and the loaded AMB was found to be in a monomeric form, even with a concentration one hundredfold higher than that usually observed for commercial AMB aqueous solutions. Loaded nanocapsules show an in vitro controlled release of AMB. As the monomeric AMB state decreased drug toxicity, ASSP nanocapsules loaded with AMB (NC1.68) have potential for use as a drug delivery system. AMB loaded NC 1.68 keeps its activity against 5 strains of Candida albicans tested. [Display omitted]