Spirulina supplementation in a mouse model of diet-induced liver fibrosis reduced the pro-inflammatory response of splenocytes

• Published in: British journal of nutrition 2019-04, Vol.121 (7), p.748-755
• Main Authors: Pham, Tho X., Lee, Yoojin, Bae, Minkyung, Hu, Siqi, Kang, Hyunju, Kim, Mi-Bo, Park, Young-Ki, Lee, Ji-Young
• Format: Article
• Language: English
• Subjects: Alanine; Alanine transaminase; Animals; Anti-Inflammatory Agents - pharmacology; Aquatic plants; Body weight; Cholesterol; Cytokines; Diet; Diet, High-Fat - adverse effects; Dietary Supplements; Disease Models, Animal; Energy expenditure; Fibrosis; Food; Gene expression; Glucose tolerance; Growth factors; High cholesterol diet; High fat diet; Hypertension; In vivo methods and tests; Inflammation; Inflammatory bowel disease; Inflammatory response; Lipids; Liver; Liver Cirrhosis - etiology; Liver Cirrhosis - prevention & control; Liver diseases; Low fat diet; Male; Mice; Mice, Inbred C57BL; Nutritional Immunology; Obesity; Obesity - complications; Rodents; Serum lipids; Spirulina; Spleen; Spleen - cytology; Splenocytes; Sucrose; Sugar; Wound healing
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1017/S0007114519000126

Treatment of liver fibrosis is very limited as there is currently no effective anti-fibrotic therapy. Spirulina platensis (SP) is a blue-green alga that is widely supplemented in healthy foods. The objective of this study was to determine whether SP supplementation can prevent obesity-induced liver fibrosis in vivo. Male C57BL/6J mice were randomly assigned to a low-fat or a high-fat (HF)/high-sucrose/high-cholesterol diet or an HF diet supplemented with 2·5 % SP (w/w) (HF/SP) for 16 or 20 weeks. There were no significant differences in body weight, activity, energy expenditure, serum lipids or glucose tolerance between mice on HF and HF/SP diets. However, plasma alanine aminotransferase level was significantly reduced by SP at 16 weeks. Expression of fibrotic markers and trichrome stains showed no differences between HF and HF/SP. Splenocytes isolated from HF/SP fed mice had lower inflammatory gene expression and cytokine secretion compared with splenocytes from HF-fed mice. SP supplementation did not attenuate HF-induced liver fibrosis. However, the expression and secretion of inflammatory genes in splenocytes were significantly reduced by SP supplementation, demonstrating the anti-inflammatory effects of SP in vivo. Although SP did not show appreciable effect on the prevention of liver fibrosis in this mouse model, it may be beneficial for other inflammatory conditions.