Characterization of Labrenzia polysiphoniae sp. nov. isolated from red alga Polysiphonia sp

A group of five Gram-negative aerobic halophilic bacteria was isolated from the red alga Polysiphonia sp. specimen collected from the Sea of Japan seashore and subjected to a taxonomic study. On the basis of 16S rRNA gene sequence analysis, the novel isolates were affiliated to the genus Labrenzia s...

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Bibliographic Details
Published in:Archives of microbiology 2019-07, Vol.201 (5), p.705-712
Main Authors: Romanenko, Lyudmila A., Kurilenko, Valeriya V., Guzev, Konstantin V., Svetashev, Vassilii I.
Format: Article
Language:English
Subjects:
Algae
Bacteria
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Deoxyribonucleic acid
DNA
Ecology
Halophilic bacteria
Hybridization
Lecithin
Life Sciences
Lipids
Microbial Ecology
Microbiology
Microorganisms
New species
Original Paper
Phosphatidylcholine
Phosphatidylethanolamine
Phosphatidylglycerol
Phospholipids
Phylogeny
Polysiphonia
rRNA 16S
Strains (organisms)
Ubiquinone
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Summary:A group of five Gram-negative aerobic halophilic bacteria was isolated from the red alga Polysiphonia sp. specimen collected from the Sea of Japan seashore and subjected to a taxonomic study. On the basis of 16S rRNA gene sequence analysis, the novel isolates were affiliated to the genus Labrenzia sharing the highest gene sequence similarities of 98.1–98.4% with the type strain of Labrenzia suaedae KACC 13772 T . The DNA–DNA hybridization values of 83–91% obtained between five novel strains, and 26 and 36% between two of the five novel strains and the closest neighbor Labrenzia suaedae KACC 13772 T confirmed their assignment to the same separate species. Novel isolates were characterized by Q-10 as the major ubiquinone, by the predominance of C 18:1 ω 7 c followed by 11-methyl C 18:1 ω 7 c and C 14:0 3-ОН in their fatty acid profiles. Polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, an unknown aminophospholipid, and an unknown phospholipid. Some of novel strains were found to inhibit growth of Gram-negative and Gram-positive test microorganisms. On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotypic traits, a novel species with the name Labrenzia polysiphoniae sp. nov. (type strain KMM 9699 T  = rh46 T  = KACC 19711 T ), is proposed.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-019-01640-0